1-deoxysphingolipids bind to COUP-TF to modulate lymphatic and cardiac cell development

ABSTRACT: We identified 1-deoxysphingosine as a ligand for NR2F1 and NR2F2. To prove physiological relevance after in vitro binding assay we used different strategies to manipulate levels of 1-deoxysphingosines and canonical sphingosines in cardiomyocytes derived from human embrynonic stem cells. These manipulations included treating cells with sphingolipid biosynthesis inhibitors (myriocin), adding 1-deoxysphingosines to culture media or induced expression of the Sptlc1 HSAN mutant C133W to enhance 1-deoxysphingosine synthesis by cardiomyocytes.

ORGANISM(S): Homo sapiens

PROVIDER: GSE184913 | GEO | 2021/10/21

REPOSITORIES: GEO