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Defining the fetal gene program at single cell resolution in dilated cardiomyopathy.

ABSTRACT: It is hypothesized that the postnatal heart adopts a fetal-like transcriptional state in response to cardiac stress. Here, we analyse the transcriptome of 74,451 nuclei from fetal, non-diseased and early-onset DCM samples, which revealed 7 broad cell clusters across fetal, ND and DCM samples. We find that there are no statistically significant shifts in cellular composition between DCM and ND hearts. Also, pseudo-bulk profiling revealed that transcriptional pathways perturbed in DCM are predominantly associated with cardiomyocytes, fibroblasts and immune cells. We advance the hypothesis that only a small subset (<10%) of fetal genes is re-engaged in both cardiomyocyte and cardiac fibroblast of DCM. This study provides a framework to identify critical gene expression networks that underpin disease pathogenesis independent of genetic aetiology.

ORGANISM(S): Homo sapiens

PROVIDER: GSE185100 | GEO | 2022/10/12

REPOSITORIES: GEO

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