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RNA sequencing of gastric epithelial cells treated with bacteria

ABSTRACT: We treated AGS cell line with Helicobacter pylori, Fusobacterium nucleatum, and Neisseria subflava, and extracted RNA after 4 hours. RNAs were applied to RNA sequencing analysis to investigate bacteria-specific effects on gastric epithelial cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE185270 | GEO | 2023/10/01

REPOSITORIES: GEO

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RNA sequencing of gastric organoids treated with bacteria

Project description:We treated mouse gastric organoids with Helicobacter pylori, Fusobacterium nucleatum, and Neisseria subflava, and extracted RNA after 4 hours. RNAs were applied to RNA sequencing analysis to investigate bacteria-specific effects on gastric epithelial cells.

2024-04-29 | GSE202509 | GEO

Gene expression profiling of gastric cells infected by Helicobacter pylori

Project description:Helicobacter pylori are gram-negative bacteria that colonize the human stomach and are the major etiological factor in gastric carcinoma development. The aim of this work was to evaluate changes in gene expression in gastric cells induced by H. pylori. The human gastric carcinoma-derived cell line AGS was infected with H. pylori strain 60190 (ATCC 49503) for 24 hours. RNA was extracted from three independent experiments.

2016-03-01 | E-GEOD-70394 | biostudies-arrayexpress

CircMAN1A2 is upregulated by Helicobacter pylori and promotes development of gastric cancer

Project description:In this study, we treated the gastric cancer cell line AGS with PBS and Helicobacter pylori to perform RNA-seq analysis. A total of 18,308 different circRNA candidates were obtained in the experiment.Compared with the control, 101 significantly differentially expressed circRNAs were identified in the AGS cells infected with H. pylori, including 84 upregulated circRNAs and 17 downregulated circRNAs.Then, circMAN1A2 with the most significant expression difference was selected according to the sequencing results to study the epigenetic mechanism of H. pylori-induced gastric carcinogenesis.

2021-09-09 | GSE183628 | GEO

Gastric cancer cells treated with exosomes derived from Fusobacterium nucleatum infected MKN-28 cells [RNA-seq]

Project description:To investigate the role of Fusobacterium nucleatum (Fn) in the development of gastric cancer, we extracted exosomes from the culture supernatant of MKN-28 cells treated with Fn or PBS, co-cultured them with AGS cells, and analyzed their RNA-seq data for gene expression profiling.

2023-06-07 | GSE214817 | GEO

Gastric cancer cells treated with exosomes derived from Fusobacterium nucleatum infected MKN-28 cells [miRNA-seq]

2023-06-07 | GSE213833 | GEO

Gene expression profiling of gastric cells infected by Helicobacter pylori

2016-03-01 | GSE70394 | GEO

Expression data from AGS cells with Helicobacter pylori infection

Project description:Helicobacter pylori (H. pylori) is a human pathogen that infects almost half of the world’s population. Infection with H. pylori is frequently associated with chronic gastritis and can even lead to gastric and duodenal ulcers and gastric cancer. Although the persistent colonization of H. pylori and the development of H. pylori-associated gastritis remain poorly understood, it is believed that, in gastric mucosa, the modulated gastric epithelial cells (GECs) by H. pylori are key contributors. We used microarrays to detail the global programme of gene expression in Helicobacter pylori infected-gastric epithelial cell line AGS cells and identified up-regulated genes induced by Helicobacter pylori infection.

2024-04-18 | GSE264263 | GEO

Gene expression changes in gastric epithelial cells induced by Helicobacter pylori infection

Project description:This group is focusing on the molecular mechanisms of the biosynthesis of glycoconjugates and carbohydrate ligands important in the process of gastric carcinogenesis and on gastric carcinoma progression and metastasis. The adhesion of H. pylori to epithelial cells induces a variety of modifications, including changes in cell morphology,cytokine production and release, alterations in glycosylation and in the biosynthesis of mucins. This study aims to identify the gene expression changes in the gastric epithelial cells (AGS) induced by Helicobacter pylori infection. This experiment was performed as previously described in the literature by submitting a gastric cell line to co-culture with reference strains of H. pylori.

2011-02-17 | GSE27347 | GEO

Gene expression changes in gastric epithelial cells induced by Helicobacter pylori infection

2011-02-17 | E-GEOD-27347 | biostudies-arrayexpress

Expression data from Helicobacter pylori isolates infecting a gastric epithelial progenitor cell line

Project description:Helicobacter pylori clinical isolates can establish themselves in gastric epithelial stem cells and this interaction may have implications for gastric tumorigenesis. Mouse gastric epithelial progenitor cells (mGEPs) were infected for 24hrs with Helicobacter pylori clinical isolates Kx1 and Kx2. Kx1 and Kx2 were also grown in cell media in the absence of cells. Kx1 was isolated from a patient with chronic atrophic gastritis (ChAG) and Kx2 from the same patient 4 years later, when he progressed to gastric adenocarcinoma. Keywords: RNA Expression Array H. pylori strains Kx1 or Kx2 that had been grown to log phase were used to infect mGEP cells.. After 24h, media and non-attached bacteria were washed off and the cells harvested by trypsinization. RNA was prepared from bacteria infecting mGEP cells or bacterial cultures of Kx1 and Kx2 grown in cell media for 24h in the absence of mGEP cells.

2010-05-26 | E-GEOD-10261 | biostudies-arrayexpress

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