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Calibrated native ChIP-seq for H2AK119ub1 and H3K27me3 in FKBP12-PCGF3/5 mESCs

ABSTRACT: X chromosome inactivation (XCI) is mediated by the non-coding RNA Xist which directs chromatin modification and gene silencing in cis. The RNA binding protein SPEN and associated corepressors have a central role in Xist-mediated gene silencing. Other silencing factors, notably the Polycomb system, have been reported to function downstream of SPEN. Making use of a SPEN separation-of-function mutation we show that SPEN and Polycomb pathways in fact function in parallel to establish gene silencing. Additionally, we find that differentiation-dependent recruitment of the chromosomal protein SmcHD1 is required for silencing many X-linked genes.

ORGANISM(S): Mus musculus

PROVIDER: GSE185769 | GEO | 2022/05/17

REPOSITORIES: GEO

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