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Drosophila 16-20 hrs embryos: DHR3G60S/Df(2R)12 vs. w1118

ABSTRACT: Transcriptional profiling of 16-20 hrs Drosophila embryos comparing control (w1118) and DHR3 mutants (DHR3G60S/Df(2R)12)

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE18577 | GEO | 2009/12/01

SECONDARY ACCESSION(S): PRJNA120283

REPOSITORIES: GEO

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Drosophila 16-20 hrs embryos: DHR3G60S/Df(2R)12 vs. w1118

Project description:Transcriptional profiling of 16-20 hrs Drosophila embryos comparing control (w1118) and DHR3 mutants (DHR3G60S/Df(2R)12) Two-condition experiment, control vs. DHR3 mutant embryos. Biological replicates: 4 replicates.

2010-05-19 | E-GEOD-18577 | biostudies-arrayexpress

Analysis of gene expression in Df(2R)ED3921 (hmgd/z deficiency) and Df(2R)ED50000 (hmgd deficiency) strains

2008-07-15 | GSE11880 | GEO

Analysis of gene expression in Df(2R)ED3921 (hmgd/z deficiency) and Df(2R)ED50000 (hmgd deficiency) strains

Project description:We analysed the effect of the deficiency Df(2R)ED3921 and Df(2R)ED50000 on gene expression in embryos (stage 0-11), and wing imaginal discs and brains from 3rd instar larvae. Df(2R)ED3921 and Df(2R)ED50000 were described in Ragab et al. (2005) Genetics 172:1069-1078 2005. RNA from Df(2R)ED3921 and Df(2R)ED50000 embryos was compared to a pool of RNA extracted from wild-type embryos at the same stage. For each genotype, 4 independent biological replicates were performed (2 of these dyes swapped with respect to the other two). The same experimental protocol and genotypes were used for the analysis in dissected wing imaginal discs and brains from 3rd instar larvae.

2008-07-15 | E-GEOD-11880 | biostudies-arrayexpress

Drosophila melanogaster

Project description:Drosophila 16-20 hrs embryos: DHR3G60S/Df(2R)12 vs. w1118

| PRJNA120283 | ENA

DNA-Seq of DrosDel deficiency lines and w1118 lines

Project description:DNASeq reads from pools of female or male DrosDel and w1118 (parental strain for the Df/+ flies). Samples are named in this dataset according to the following sample naming scheme: tissue_genotype shorthand_sex_DNASeq_biological replicate #. We sequenced DNA from pools of female or male DrosDel and w1118 (parental strain for the Df/+ flies).

2012-04-03 | E-GEOD-31550 | biostudies-arrayexpress

DNA-Seq of DrosDel deficiency lines and w1118 lines

2012-04-03 | GSE31550 | GEO

Project description:df

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RNA-Seq on SOLiD platform of DrosDel deficiency and w1118 flies

Project description:RNA-Seq reads of DrosDel deficiency (Df/+) and parental strain diploid flies along with spike-in controls was performed on the SOLiD platform. Samples are named in this dataset according to the following sample naming scheme: tissue_genotype shorthand_sex_biological replicate #_platform_technical replicate. We sequenced mRNA from pools of female or male DrosDel and w1118 (parental strain for the Df/+ flies). The same sample was sequenced across two flow cells on the SOLiD platform.

2012-04-03 | E-GEOD-31401 | biostudies-arrayexpress

Streptomyces sp. 2R strain:2R

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Transcription profiling of mouse Df(16)A/+ and wild type littermates

Project description:This represents an unbiased evaluation of the transcriptional response in the prefrontal cortex and hippocampus areas in the Df(16)A/+ mice, a mouse model of human 22q11 microdeletion syndrome. These mice were generated by chromosomal engineering and carry a microdeltion of ~1.3Mb in the mouse locus syntenic to the human 22q11.1; The reasoning behind this expression profiling is that alterations in transcriptional programs reflect either downstream (immediate or remote) effects of the deficiency or reactive (compensatory) changes, and can thus point to affected biological processes and molecular functions. Experiment Overall Design: A total of 20 PFC and 20 HPC from 10 Df(16)A/+ mutants and 10 WT control mice was used for RNA extraction and hybridization on Affymetrix microarrays. All mice were male littermates 8 weeks of age.

2008-06-11 | E-GEOD-10784 | biostudies-arrayexpress

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