Transcriptomics

Dataset Information

18

Estrogen Coordinates Translation and Transcription Revealing a Role for NRSF in Human Breast Cancer Cells


ABSTRACT: Analysis of estrogen receptor (ER)-positive MCF7 cell total RNA expression and polysome-assiciated RNA expression following treatment with estradiol (E2) and vehicle (etoh). We used expression microarrays to measure polysome association and total RNA abundance in E2 treated MCF7. These data, along with previously published data, show that genes that are upregulated by estrogen treatment are biased towards association with polysomes. Overall design: MCF7 cells were grown in hormone depleted media for three days before a 1 hour treatment with E2 or 0.1 % ethanol (vehicle). Total RNA was collected using standard methods and polysome-association RNA from the same cells were collected using sucrose gradient fractionation. Both RNA populations were purified, labeled, and hybridized to Affymetrix Human Genest arrays.

INSTRUMENT(S): [HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version]

ORGANISM(S): Homo sapiens  

SUBMITTER: Alexander Brodsky   

PROVIDER: GSE18592 | GEO | 2010-04-21

SECONDARY ACCESSION(S): PRJNA121331

REPOSITORIES: GEO

altmetric image

Publications

Sorry, this publication's infomation has not been loaded in the Indexer, please go directly to PUBMED or Altmetric.

Similar Datasets

2012-03-27 | E-GEOD-18592 | ArrayExpress
2012-03-27 | E-GEOD-17460 | ArrayExpress
2009-08-04 | GSE17460 | GEO
| GSE8139 | GEO
| GSE8140 | GEO
| GSE22012 | GEO
2011-06-10 | E-GEOD-8139 | ArrayExpress
2011-06-10 | E-GEOD-8140 | ArrayExpress
2012-03-06 | E-GEOD-8141 | ArrayExpress
2008-12-01 | GSE8141 | GEO