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Pseudomonas aeruginosa gene regulation by Staphylococcus aureus exoproducts

ABSTRACT: Pseudomonas aeruginosa and Staphylococcus aureus are often co-isolated in persistent infections. The goal of this study was to determine how secreted products from S. aureus affect gene expression in P. aeruginosa. Therefore, media control or S. aureus supernatant was added to P. aeruginosa cultures at 25% total volume and gene expression was measured at 20 min, 1 h, and 2 h using RNA-seq. Overall, after addition of S. aureus supernatant, there was an upregulation in genes involved in metal deprivation and intermediate metabolite uptake.

ORGANISM(S): Pseudomonas aeruginosa

PROVIDER: GSE185963 | GEO | 2022/05/18

REPOSITORIES: GEO

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Pseudomonas aeruginosa gene regulation by Staphylococcus aureus supernatant or added secreted products

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Surface-associated, motile populations of Pseudomonas aeruginosa gene regulation by Staphylococcus aureus exoproducts

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2024-01-18 | E-MTAB-12581 | biostudies-arrayexpress

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The innate immune protein calprotectin promotes Pseudomonas aeruginosa and Staphylococcus aureus interaction

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2016-09-29 | GSE81065 | GEO

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2021-09-09 | PXD023445 | Pride

Transcriptional profiling of Pseudomonas aeruginosa and Staphylococcus aureus during in vitro co-culture

Project description:We analyzed the transcriptome of both S. aureus and P. aeruginosa after 3h of co-culture

2018-11-01 | GSE122048 | GEO

Untargeted metabolomics reveals species-specific metabolite production and shared nutrient consumption by Pseudomonas aeruginosa and Staphylococcus aureus

Project description:While bacterial metabolism is known to impact antibiotic efficacy and virulence, the metabolic capacities of individual microbes in cystic fibrosis lung infections are difficult to disentangle from sputum samples. Here, we show that untargeted metabolomic profiling of supernatants of multiple strains of Pseudomonas aeruginosa and Staphylococcus aureus grown in monoculture in synthetic cystic fibrosis media (SCFM) reveal distinct species-specific metabolic signatures with limited strain-to-strain variability. The majority of metabolites significantly consumed by S. aureus were also consumed by P. aeruginosa, indicating that P. aeruginosa has the flexibility to metabolically outcompete S. aureus in coculture even in the absence of other pathogen-pathogen interactions. Finally, metabolites that were uniquely produced by one species or the other were identified. Specifically, the virulence factor precursor anthranilic acid as well as the quinoline 2,4-Quinolinediol (DHQ) were robustly produced across all tested strains of P. aeruginosa. Through the direct comparison of the extracellular metabolism of P. aeruginosa and S. aureus in a physiologically relevant environment, this work provides insight towards the potential metabolic interactions in vivo and supports the development of species-specific diagnostic markers of infection.

2021-05-26 | MTBLS2105 | MetaboLights

Tn-seq of Staphylococcus aureus exposed to Calprotectin

Project description:The host protein calprotectin inhibits the growth of a variety of bacterial pathogens through metal sequestration in a process known as 'nutritional immunity'. Staphylococcus aureus growth is inhibited by calprotectin in vitro and calprotectin is localized in vivo to staphylococcal abscesses during infection. However, the staphylococcal adaptations that provide defense against nutritional immunity and the role of metal-responsive regulators are not fully characterized. In this work, we define the transcriptional response of S. aureus and the role of the metal-responsive regulators, Zur, Fur, and MntR, in response to metal limitation by calprotectin exposure. Additionally, we identified genes affecting the fitness of S. aureus during metal limitation through a Transposon sequencing (Tn-seq) approach. Loss of function mutations in clpP, which encodes a proteolytic subunit of the ATP-dependent Clp protease, demonstrate reduced fitness of S. aureus to the presence of calprotectin. ClpP contributes to pathogenesis in vivo in a calprotectin-dependent manner. These studies establish a critical role for ClpP to combat metal limitation by calprotectin and reveal the genes required for S. aureus to outcompete the host for metals.

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