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VAL1/2 recruit PKL to target sites in arabidopsis genome

ABSTRACT: PICKLE (PKL), a Chromodomain Helicase DNA binding domain type 3-type (CHD3) chromatin remodeler, noted for an embryonic structure called pickle root in primary root tip in pkl mutant, has been studied for decades. we obtained a comprehensive genome occupancy of PKL by Chromatin immunoprecipitation-sequencing (ChIP-seq), and found PKL co-occupy with the major repressors of seed maturation program, VIVIPAROUS1/ABI3-LIKE1/2 (VAL1/2) in genome. Furthermore, PKL physically interacts with VAL1/2 in vivo and phenotype and transcriptome data indicated that PKL and VAL1/2 function in a common pathway. Moreover, ChIP-seq and ChIP-qPCR results showed that VAL1/2 are necessary for the recruitment of PKL to co-target genes

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE186157 | GEO | 2023/05/10

REPOSITORIES: GEO

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The Transcriptional Repressors VAL1 and VAL2 Recruit PRC2 for Genome-wide Polycomb Silencing in Arabidopsis

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Identify differentially expressed genes in 14-day-old pkl seedlings

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Next Generation Sequencing Facilitates Quantitative Analysis of in 14-day-old wt and val1 val2 pkl seedlings.

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