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Identification of Aurora B pathway involved in the anti-cancer effects by Paeoniae Radix extracts based on whole transcriptome analysis

ABSTRACT: we profiled the change in genome-wide gene expression of human lung cancer cells in response to treatment of water and ethanol extracts of PR and its ingredients in a dose dependent manner to investigate PR efficacy at the molecular pathway level.

ORGANISM(S): Homo sapiens

PROVIDER: GSE186218 | GEO | 2022/12/31

REPOSITORIES: GEO

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High-throughput transcriptomics of water extracts detects reductions in biological activity with water treatment processes

Project description:The presence of numerous chemical contaminants from industrial, agricultural, and pharmaceutical sources in water supplies poses a potential risk to human and ecological health. Current chemical analyses suffer from limitations including chemical coverage and high cost, and broad-coverage in vitro assays such as transcriptomics may further improve water quality monitoring by assessing a large range of possible effects. Here, we used high-throughput transcriptomics to assess the activity induced by field-derived water extracts in MCF7 breast carcinoma cells.

2024-03-05 | GSE252117 | GEO

Identification of the pharmacological effects of Chrysanthemum morifolium Ramat. leaves extracts on high-fat diet induced obese mice through lipid-, and glucose metabolism

Project description:We investigated that ethanol extracts of Chrysanthemum morifolium Ramat. leaves improved metabolic disease on high-fat diet induced obese mice using metabolic profiling of lipid-, glucose, inflammation and RNA-seq transcriptomic profling.

2019-04-24 | GSE130218 | GEO

1H NMR spectroscopy-based metabolic profiling of Ophiocordyceps sinensis and Cordyceps militaris of water-boiled and 50% ethanol-soaked extracts

Project description:Introduction Ophiocordyceps sinensis, a well-known Chinese complementary herb, is a rare and valuable therapeutic resource. Cordyceps militaris (C. militaris) is a commonly used substitute for O. sinensis. A metabolomic-based approach for exploring the similarities and differences in the metabolites of O. sinensis and C. militaris in water-boiled and 50% ethanol-soaked extracts is of great significance. Objectives To determine a vital role of extraction methodologies in influencing the metabolic composition of herbs, 1HNMR-based profiling was used to characterize the metabolic fingerprints of O. sinensis and C. militaris. Methods To make a distinction between the global metabolite profiling of O. sinensis and C. militaris extracts obtained from either the water-boiled or 50% ethanol-soaked methods, we screened the herbs samples using 1HNMR-based metabolic fingerprints combined with multivariate statistical analysis. Results This study revealed that a total of 43 (82.69% of 52) metabolites were detectable in both O. sinensis and C. militaris. According to the variable importance in projection (VIP) value and p-value from the Mann-Whitney test, 7 metabolites (alanine, aspartate, glutamate, mannitol, ornithine, serine, and trehalose) differed between O. sinensis and C. militaris. Arginine, glucose, putrescine, pyroglutamate, betaine, O-phosphocholine, and xylose differed significantly between the water-boiled and 50% ethanol-soaked methods used to prepare the herb extracts. Conclusion A total of 52 primary metabolites were identified and quantified from O. sinensis and C. militaris samples. The study suggests that a water-boiled extraction is much faster method and strongly recommended over the 50% ethanol-soaked method for both O. sinensis and C. militaris.

2019-04-28 | ST001189 | MetabolomicsWorkbench

Gene expression profiles of human mesenchymal stem cells exposed to surface water organic extracts

Project description:To assess the impact of surface water across the Hun River, several sampling sites located in the mainstream and the tributary were selected representative of pollution gradient and different pollution source. Human mesenchymal stem cells were exposed to organic extracts of surface water from six sites for 2 days. Microarrays were used to measure the gene expression. And the gene expression profiles were used to evaluate the ability of determine the potential biological effects, to differentiate different pollution source, and to identify the toxic components.

2018-06-01 | GSE110310 | GEO

the anti lipid accumulation and anti liver cancer formation effect of CACF in CD36 overexpression transgenic zebrafish

Project description:Carassius auratus complex formula (CACF) is composed of extracts from Carassius auratus, Rhizoma dioscoreae, Lycium chinense, and Rehmannia glutinosa Libosch. These ingredients are used to treat diabetes since ancient China. Previously, CACF elicits antidiabetic effects in streptozotocin-induced diabetic mouse models under high-fat diet treatment, reduces triglyceride levels, maintains blood sugar control, and enhances insulin sensitivity. However, the effects of CACF on anti-cancer and anti-inflammation and the underlying mechanism remain unknown. In this study, we revealed that CACF could be a promising therapeutic agent with multiple functions

2023-01-31 | GSE217998 | GEO

Proteomics analysis of the effects of Grammatophyllum speciosum extracts on macrophage cells

Project description:Grammatophyllum speciosum is a traditional plant with beneficial functionalities for health. G. speciosum extracts can inhibit collagenase and nitric oxide without cellular toxicity in keratinocytes. The extracts have shown potential for use and formulation as cosmeceutical ingredients. However, the molecular mechanisms underlying these activities remain unknown. In this dataset, we used a proteomics approach to clarify the proteins that participate in the response of RAW264.7 macrophage cells to G. speciosum extracts. Cells were divided into two experimental groups, i.e., the control and treatment groups. In turn, the treatment group included two subgroups that were treated with 20 and 100 g/mL of the extracts, respectively. The experiments were conducted using two biological replicates. The dataset was obtained from label-free proteomics using high-resolution LC-MS/MS with four technical replicates. The quality control (QC) of the proteomics dataset was carried out using chromatography at the MS1 and MS2 levels, peptide mass deviation, peptide mass cleavage, sequence length, and total peptide intensity.

2023-01-03 | PXD039206 | Pride

Anti-fatigure properties of Sasa quelpaertensis leaf extracts in mice

Project description:Anti-fatigure properties of Sasa quelpaertensis leaf extracts in mice

| PRJNA453928 | ENA

Proteomics analysis of the effects of Grammatophyllum speciosum extracts on RAW 264.7 cells

2022-12-27 | PXD038611 | Pride

The Study of Gene Expression Profiles of KG-1 Cells Treated with Antrodia Camphorata Extracts using cDNA microarray.

Project description:Antrodia Camphorata is well known in Taiwan as a folk medicinal fungus with anticancer and anti-inflammatory effects. In this study, we used a human acute myelogenous leukemia cell line, KG-1, as the experimental model and constructed a high-throughput gene screen integrated platform by using a combination of herbal identification, UV-VIS spectrophotometry, high performance liquid chromatography (HPLC), inductively coupled plasma mass spectrometry (ICPMS), and DNA microarray technology. Based on this science-based platform, we developed a practical quantitative method and also established an organic/inorganic chemical fingerprint for the herbal materials. In the mean while, the global gene expression patterns of the KG-1 cells treated with various A. camphorata mycelia extracts (water and methanol extracts) were compared. Furthermore, several possible biological responses and relevant marker gene would be identified by our high-throughput integrated platform. Keywords: KG-1 treated with AC extracts for 4 days, cDNA microarray, biomarker selection.

2008-11-18 | GSE3692 | GEO

Integrated analysis reveals effects of bioactive ingredients from Limonium Sinense (Girard) Kuntze on hypoxia-inducible factor (HIF) activation

Project description:Limonium Sinense (Girard) Kuntze is a traditional Chinese medicinal herb, showing blood replenishment, anti-tumour, anti-hepatitis, and immunomodulation activities amongst others. However, the mechanism of its pharmacological activities remains largely unknown. Here, we investigated the effects of bioactive ingredients from Limonium Sinense using integrated analysis. Water extracts from Limonium Sinense (LSW) showed a strong growth inhibitory effect on multiple cells in both 2D and 3D cultures. Global transcriptomic profiling and further connectivity map (CMap) analysis identified several similarly acting therapeutic candidates, including Tubulin inhibitors and hypoxia-inducible factor (HIF) modulators. The effect of LSW on cell cycle was verified with flow cytometry showing a G2/M phase arrest. Integrated analysis suggested a role for gallic acid in mediating HIF activation. Taken together, this study provides novel insights into the bioactive ingredients in Limonium Sinense, highlighting the rich natural resource and therapeutic values of herbal plants.

2022-09-29 | GSE205252 | GEO

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