Transcriptomics,Genomics

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Transcriptome-wide effects of NusA on RNA polymerase pausing in Bacillus subtilis


ABSTRACT: In this work we conducted RNET-seq on Wild Type, NusA depletion, nusG deletion, and NusA depletion nusG deletion strains of B. subtilis. Using this data we analysed the transcriptome-wide effects of NusA on RNA polymerase pausing and found that NusA has modest pause stimulating as well as suppressing effects throughout the genome. Overall design: RNET-seq on Wild Type, NusA depletion, nusG deletion, and NusA depletion nusG deletion strains of B. subtilis followed by in vitro biochemistry follow-up.

INSTRUMENT(S): Illumina HiSeq 2000 (Bacillus subtilis)

ORGANISM(S): Bacillus Subtilis

SUBMITTER: Oshadhi Jayasinghe  

PROVIDER: GSE186285 | GEO | 2021-10-22

REPOSITORIES: GEO

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