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Transcriptome-wide effects of NusA on RNA polymerase pausing in Bacillus subtilis

ABSTRACT: In this work we conducted RNET-seq on Wild Type, NusA depletion, nusG deletion, and NusA depletion nusG deletion strains of B. subtilis. Using this data we analysed the transcriptome-wide effects of NusA on RNA polymerase pausing and found that NusA has modest pause stimulating as well as suppressing effects throughout the genome.

ORGANISM(S): Bacillus subtilis

PROVIDER: GSE186285 | GEO | 2021/10/22

REPOSITORIES: GEO

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NusG is an intrinsic transcription termination factor that stimulates motility and cooperatively coordinates global gene expression with NusA

Project description:In this work we conducted Term-seq on Wild Type, NusA depletion, nusG deletion, and NusA depletion nusG deletion strains of B. subtilis. Using this approach, we found that NusG is an intrinsic termination factor that works single and cooperatively with NusA.

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2016-07-03 | E-GEOD-67058 | biostudies-arrayexpress

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Ribosome profiling reveals conserved guanosine residues at ribosome pausing sites in Bacillus subtilis [Ribo-seq]

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Nascent elongating transcript sequencing (NET-seq) for Escherichia coli and Bacillus subtilis reveals a consensus pause sequence enriched at translation start sites.

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2014-05-05 | E-GEOD-56720 | biostudies-arrayexpress

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