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Mitochondrial Ca2+ homeostasis controls inter-compartmental proteostasis in Arabidopsis plants

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE186418 | GEO | 2026/06/03

REPOSITORIES: GEO

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Mitochondrial Ca2+ homeostasis controls inter-compartmental proteostasis in Arabidopsis plants

Project description:The goals of this study are to determine effects of mitochondrial Ca2+ homeostasis on inter-compartmental proteostasis

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Mitochondrial Ca2+ homeostasis controls inter-compartmental proteostasis in Arabidopsis plants [mRNA]

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Mitochondrial Ca2+ homeostasis controls inter-compartmental proteostasis in Arabidopsis plants [Ribo-seq]

Project description:Purpose: The goals of this study are to determine the effects of mitochondrial Ca2+ homeostasis on cytosolic translation Methods: The monosomal and polysomal mRNA profiles of 10-day-old WT, MCU2 overexpression and mcu sextuple mutation plants were generated by deep sequencing, in triplicate, using Illumina HiSeq2500. The sequence reads that passed quality filters were analyzed at the transcript isoform level with TopHat followed by Cufflinks Results: Using an optimized data analysis workflow, we mapped a minimum of 23 million sequence reads per sample to the Arabidopsis genome (TAIR10) and identified more than 23,000 transcripts. Data analysis revealed that perturbation of mitochondrial Ca2+ altered specifically the translational efficiency of RNA modification genes Conclusions: Our study represents the first detailed analysis of Arabidopsis monosomal and polysomal RNA transcriptomes in response to impaired mitochondrial Ca2+, with biologic replicates, generated by RNA-seq technology. Our results showed that perturbation of mitochondrial Ca2+ triggered cytosolic translation response. We conclude that RNA-seq based transcriptome characterization would expedite genetic network analyses of inter-compartmental communication

2026-06-03 | GSE186417 | GEO