Evaluating hormone responses in Esr1-wildtype and mutant mammary luminal cells [RNA-seq]

ABSTRACT: To examine the effects of hormone stimulation on Esr1-wildtype and Esr1-mutant mammary glands, we will ovariectomize both wildtype and ER-mutant mice. Following ovariectomy (OVX), animals will be implanted with hormone pellets (either placebo, estrogen, and/or progesterone). Then, we perform RNA-seq on FACS-sorted mature luminal cells (ML) from mammary glands of treated mice. This analysis will allow for separation of ER mutant-intrinsic effects on gene expression vs. combinatorial effects from exposure to either estrogen or progesterone. 

ORGANISM(S): Mus musculus

PROVIDER: GSE186453 | GEO | 2023/05/07

REPOSITORIES: GEO