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Expression data of human perirenal adipose tissue-derived mesenchymal stem cells cultured under various conditions

ABSTRACT: Human mesenchymal stem cells (MSC) derived from perirenal adipose tissue (PV) of living kidney donors were cultured under various conditions, namely (1) control (medium+foetal bovine serum(FBS)) or (2) control (medium+heat-inactivated FBS); (3) with mixed-lympohocyte reactions (MLR) in transwell culture systems for 4 days; (4) with mixed-lympohocyte reactions (MLR) in transwell culture systems for 7 days; or (5)with pro-inflammatory cytokines(IFNgamma, TNFalpha and interleukin 6). We used microarrays to detail the effect of the various culture conditions on MSC.

ORGANISM(S): Homo sapiens

PROVIDER: GSE18662 | GEO | 2010/10/01

SECONDARY ACCESSION(S): PRJNA121527

REPOSITORIES: GEO

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Expression data of human perirenal adipose tissue-derived mesenchymal stem cells cultured under various conditions

Project description:Human mesenchymal stem cells (MSC) derived from perirenal adipose tissue (PV) of living kidney donors were cultured under various conditions, namely (1) control (medium+foetal bovine serum(FBS)) or (2) control (medium+heat-inactivated FBS); (3) with mixed-lympohocyte reactions (MLR) in transwell culture systems for 4 days; (4) with mixed-lympohocyte reactions (MLR) in transwell culture systems for 7 days; or (5)with pro-inflammatory cytokines(IFNgamma, TNFalpha and interleukin 6). We used microarrays to detail the effect of the various culture conditions on MSC. MSC were cultured under control conditions (n=4 in FBS and n=3 in heat-inactivated FBS), with MLR in transwell culture systems for 4 days (n=3) and 7 days (n=4), and with pro-inflammatory cytokines(IFNgamma, TNFalpha and interleukin 6)(n=4) and collected for gene analysis

2010-10-01 | E-GEOD-18662 | biostudies-arrayexpress

Human perirenal adipose tissue

Project description:Analysis of perirenal adipose tissue from healthy kidney donors (age 44±9 years, BMI 25.8±3.3 kg/m2, mean±SD). The samples were taken bylaparoscopic technique beneath the incision plane that was created in the renal fascia (Greotas fascia) close to the renal vein. Ten samples of human perirenal adipose tissue

2014-07-11 | E-GEOD-59325 | biostudies-arrayexpress

Suckling lamb perirenal fat transcriptome

Project description:The aim of this study was to perform a comparative analysis of the perirenal fat transcriptomes of suckling lambs from two breeds with different growth and carcass characteristics. The perirenal fat tissue from 14 suckling lambs (Assaf= 8, Churra= 6) was used for the RNA-Seq analysis. The functional enrichment analysis of the 670 highly expressed genes (>150 fragments per kilobase of exon per million fragments mapped) in the perirenal fat transcriptome of both breeds revealed that the majority of these genes were involved in energy processes. The differential expression analysis performed identified 373 differentially expressed genes (DEGs) between the two compared breeds. In Assaf lambs, DEGs were enriched in Gene Ontology (GO) biological processes related to fatty-acid oxidation, while in Churra lambs, the majority of the significantly enriched GO terms were related to cholesterol synthesis, which suggests that upregulated DEGs in Assaf lambs are implicated in fat burning, while the Churra upregulated DEGs are linked to fat accumulation.

2018-07-18 | GSE108314 | GEO

Human perirenal adipose tissue

2014-07-11 | GSE59325 | GEO

Suckling lamb perirenal fat transcriptome

Project description:Suckling lamb perirenal fat transcriptome

| PRJNA423080 | ENA

Expression data from human adipose stem cells expanded in allogeneic human serum and fetal bovine serum

Project description:Human adipose stem cells (ASCs) have been shown, in pre-clinical studies, to have therapeutic applicability in diverse fields, but a standard expansion method for clinical applications remains yet to be established. Isolated ASCs are typically expanded in medium containing fetal bovine serum (FBS). However, sera and other animal-derived culture reagents stage numerous safety issues in clinical therapy, including possible infections and severe immune reactions. By expanding the ASCs in medium containing human serum (HS), the problem can be eliminated. To define how allogeneic HS performs in ASC expansion compared to FBS, we used microarrays to explore differences in gene expression between human adipose stem cells expanded in allogeneic human serum and fetal bovine serum.

2010-04-01 | GSE17090 | GEO

RNA-Seq of perirenal adipose tissue of Assaf suckling lambs

Project description:Samples of perirenal fat tissue from 8 Assaf breed suckling lambs. These animals were selected from a larger group of 17 Assaf suckling lambs for which carcass traits were measured. The 8 selected lambs were those showing the highest and the lowest values, from the larger group, for the percentage of perirenal and cavitary fat relative to the half carcass weight. Hence, considering the values for this trait, we defined the High-PF group (n = 4; average: 3.23 ± 0,.47) and the Low-PF group (n = 4; 1.65 ± 0,.16), respectively.

2023-04-25 | E-MTAB-11839 | biostudies-arrayexpress

Gene transcription in ovine fetal perirenal adipose tissue.

Project description:Next Generation Sequencing (NGS) was used to measure the levels of gene transcription in perirenal adipose tissue in late gestation sheep fetuses (~ 2 weeks before birth). Through the discovery of SNP in the population, allele specific expression was identified.

2017-03-31 | GSE79143 | GEO

Transcriptomic signature induced in bone marrow Mesenchymal Stromal Cells (MSC) after interaction with malignant multiple myeloma cells: implications in myeloma progression.

Project description:Mesenchymal stromal cells (MSCs) derived from the BM of healthy donors (dMSCs) and myeloma patients (pMSCs) were co-cultured with the model myeloma cell line - MM.1S -, and the gene expression profile of MSCs induced by this interaction was analyzed using high density oligonucleotide microarrays. Deregulated genes in co-culture common to both d/pMSCs revealed functional involvement in tumor microenvironment cross-talk, myeloma growth induction and drug resistance, angiogenesis and signals for osteoclast activation and inhibition of osteoblasts. Additional genes induced by co-culture were exclusively deregulated in pMSCs and were predominantly associated to RNA processing, the ubiquitine-proteasome pathway, regulation of cell cycle and Wnt signaling. Primary MSCs from bone marrow (BM) samples of healthy donors (n=8) and multiple myeloma (MM) patients (n=13) were generated as described by Garayoa et al. 2009 (PMID:19357701). Ficoll-Paque density gradient separation medium was used to isolate mononuclear cells from BM aspirates. MSCs were selected by adherence to plastic and subsequently expanded until passage 2 when they were used for co-culture. For the MSC-MM.1S co-cultures we used a 6-well format transwell system with 1 M-BM-5m pore size membrane. Briefly, 2 X 10^4 MSCs were first cultured attached to the lower side of the membrane, and when reached M-bM-^IM-^H 85% confluence 1 X 10^6 MM.1S cells were seeded on the upper side. After 24 h co-culture in RPMI 1640 medium supplemented with 10% FBS and antibiotics, MSCs were recovered by trypsinization, washed once in PBS and stored at -80M-BM-0C in RLT buffer (Qiagen) until RNA extraction. Absence of MM.1S cells in the recovered MSC population was assessed in selected samples by flow cytometry analysis.

2014-11-23 | E-GEOD-46053 | biostudies-arrayexpress

Transcription profiling of rat Perirenal and epididymal preadipocytes isolated from old and young individuals

Project description:Inherent depot- and age-dependent preadipocyte characteristics may contribute to age-related fat redistribution. Both aging and depot origin affect preadipocyte replication and adipogenesis. To define responsible mechanisms, we analyzed genome-wide expression profiles in epididymal (E) and perirenal (P) preadipocytes cultured from young (3 month) and old (30m) rats. Differences between depots were distinct from and more dramatic than those that occur with aging. Experiment Overall Design: Preadipocytes were isolated from perirenal and epididymal fat depots of 3 young (3 month) and 3 old (30 month) Brown Norway rats for a total of 12 samples from 6 different animals.

2008-06-15 | E-GEOD-6699 | biostudies-arrayexpress

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