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Determination of human DNA replication origin position and efficiency reveals principles of initiation zone organisation

ABSTRACT: We describe a modification of initiation site sequencing (ini-seq, Langley et al., NAR 2016) in which density gradient centrifugation separates ‘heavy’ replicated DNA containing BrdU from DNA containing only ‘light’ dTTP before sequencing. This approach allows us to assign a replication initiation efficiency score to each of the 23905 origins identified. We also performed Short Nascent Strand sequencing (SNS-seq), an established method to map replication origins, in the same cell line as in Ini-seq2 for comparison.

ORGANISM(S): Homo sapiens

PROVIDER: GSE186675 | GEO | 2022/06/15

REPOSITORIES: GEO

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