Transcriptomics

Dataset Information

50

Analysis of murine hematopoieitic stem cells, multipotent progenitors, PreMegE progenitors and mature CD4+ T cells


ABSTRACT: An investigation of the global gene expression signatures of murine hematopoietic stem cell differentiation during steady state hematopoiesis. This data compliments the miniChIP-chip data obtained from the same cell types as described in Weishaupt et al., 2009 (Blood). Overall design: An Affymetrix genechip study using total RNA recovered from three separate FACS isolated HSC and MPP samples. The HSC and MPP gene expression data was analyzed alongside the PreMegEs and CD4+ T cells datasets obtained from our previous work (Pronk et al., 2007, Rolf et al. 2008). HSCs are phenotypically identified in bone marrow as lineage-, cKit+, Sca1+, CD150+, Flk2/Flt3- (LSKCD150+ cells). MPPs are phenotypically identified in bone marrow as lineage-, cKit+, Sca1+, CD150-, Flk2/Flt3+ (LSKCD150- cells). PreMegEs are phenotypically identified in bone marrow as lineage-, cKit+, Sca1-, CD150+, CD105- and CD41- as described in Pronk et al., 2007. Splenic-derived CD4+ T cells are phentypically identified as CD4+, CD8-, B220-, Nk1.1- cells as described in Rolf et al., 2008.

INSTRUMENT(S): [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array

ORGANISM(S): Mus musculus  

SUBMITTER: Joanne Attema  

PROVIDER: GSE18669 | GEO |

SECONDARY ACCESSION(S): PRJNA123807

REPOSITORIES: GEO

Similar Datasets

2009-11-09 | E-GEOD-18669 | ArrayExpress
2009-11-09 | GSE18734 | GEO
2009-11-08 | E-GEOD-18734 | ArrayExpress
2015-05-05 | E-GEOD-68529 | ArrayExpress
2013-02-14 | E-GEOD-43729 | ArrayExpress
| GSE48893 | GEO
| GSE32265 | GEO
2012-09-19 | E-GEOD-32265 | ArrayExpress
| GSE60587 | GEO
| GSE86526 | GEO