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Transcriptome profile of Stage 40 Oryzias latipes (Medaka) embryos II

ABSTRACT: In order to identify the effects of the absence of SEDL on Medaka transcriptome, we performed RNAseq experiments Transcriptome analysis of the liver of mice trated with CD95 activating antibody, compared with liver of mice trated with saline.

ORGANISM(S): Oryzias latipes

PROVIDER: GSE186769 | GEO | 2024/05/03

REPOSITORIES: GEO

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Transcriptome profile of Stage 40 Oryzias latipes (Medaka) embryos

Project description:Transcriptomic profile of Stage 40 Oryzias latipes (Medaka) embryos wild type and KO for SEDL

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Transcriptome profile of liver tissue in CD95 activating antibody-treated mice

Project description:In order to identify the effects of CD95 activating antibody on the transcriptome of liver tissue, we performed RNAseq experiments Transcriptome analysis of the liver of mice trated with CD95 activating antibody, compared with liver of mice trated with saline.

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Transcriptomic profiles of Japanese medaka (Oryzias latipes) in response to alkalinity stress

Project description:Alkalinity stress is considered to be one of the major stressors for fish in saline-alkali water. Thus, it is of great significance from both aquaculture and physiological viewpoint to understand the molecular genetic response of aquatic organisms to alkalinity stress. The objective of this study is to determine genome-wide gene expression profiles to better understand the physiology response of medaka (Oryzias latipes) to high carbonate alkalinity stress. In lab-based cultures, adult fish were exposed to freshwater and high carbonate alkalinity water .We designed a microarray containing 26429 oligonucleotides and describe our experimental results for measuring gene expression changes in the gill of carbonate alkalinity stress exposed fish.

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Transcriptomic profiles of Japanese medaka (Oryzias latipes) in response to alkalinity stress

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Gene expression of brains (ventral part of telenchephalon, hypothalamus and pituitary) in wild type and olvl28m13 knockout Medaka fish (Oryzias latipes)

Project description:To examine the transcriptome, we compared the gene expression of brains (ventral part of telenchephalon, hypothalamus and pituitary) between wild type medaka and olvl28m13 knockout medaka.

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Seasonal changes of gene expression of eyes in Medaka fish (Oryzias latipes)

Project description:To examine the seasonal adaptation, we compared the gene expression of eyes between SL (short-day and low-temperature conditions: 10 h light/14 h dark and 8 °C) and LD (long-day and warm-temperature conditions: 14 h light/10 h dark and 26 °C) conditions in Medaka fish (Oryzias latipes).

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Variation of the genomic expression pattern after exposed to 17beta-estradiol in Japanese medaka (Oryzias latipes)

Project description:We assess gene expression patterns upon 17beta-estradiol (E2) exposure to Japanese medaka (Oryzias latipes) in order to appere the E2 effects using DNA microarray analysis. Larval medaka were exposed to 0, 3, 30 and 100 ng/L of E2 and concentration-dependent changes in gene expressions were examined using Agilent medaka DNA microarrays. At 7 day, fish were sacrificed and mRNA was extracted for gene expression analysis. In an effort to link gene expression changes to effects on higher levels of biological organization, sex characteristics, gonadal histology, GSI, and egg production and fertility were examined. In microarray experiments, the correlation factors between the controls were from 0.91 to 1.00 among control samples. We observed highly induced O. latipes Gene Indices (OLGI) related to egg-yolk protein such as vitellogenin and L-SF precursor etc., which were significantly affected in a concentration-dependent manner by E2 exposure. To clarify the function of expressed genes by E2 treatments, we selected statistically expressed genes from the microarray experiments. We found 190 genes and 72 genes which were statistically expressed in E2 treatment as induced and repressed genes, respectively. In the induced gene list, there were characteristic induced-genes in the categories of lipid metabolism, stress (oxidative stress, DNA and protein damage), and apoptosis with MAPK pathway. On the other hands, there were characteristic repressed genes in the categories of heat shock protein. Our result may suggest that gene expressions in yolk medaka is able to be used for detection of E2 effect by DNA microarray analysis. Larval medaka were exposed to 0, 3, 30 and 100 ng/L of E2 and concentration and time-dependent changes in gene expressions were examined using Agilent medaka DNA microarrays. At 7 day, three independent samples (one sample contained thirty whole medakas) were sacrificed and mRNA was extracted for gene expression analysis.

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Immunoglobulin light chains in medaka (Oryzias latipes).

Project description: Not available

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Immunoglobulin heavy chains in medaka (Oryzias latipes).

Project description: Not available

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Transcriptomic characterization of transcription activator-like effector nucleases-induced XY dmy- sex reversal female medaka (Oryzias latipes)

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2017-02-11 | GSE62700 | GEO

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