Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Preliminary investigation of the effect of transfected HBx on the expression pattern of circRNAs in hepatocellular carcinoma

ABSTRACT: Preliminary investigation of the effect of transfected HBx on the expression pattern of circRNAs in hepatocellular carcinoma

ORGANISM(S): Homo sapiens

PROVIDER: GSE186884 | GEO | 2022/10/31

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Wild-type versus HBx-transfected mive

Project description:Hepatitis B virus (HBV) has been clearly recognized as an etiological factor for hepatocellular carcinoma (HCC). HBV encodes the potentially oncogenic HBx protein. We aimed to elucidate the molecular mechanism of HCC caused by HBx and to discover the biomarker related to HCC by HBx. Three experimental groups, 3, 9 and 13 month aged HBx Tg mice and age matched normal wild type B6 mouse which have same background of HBx Tg mice were used to find differentially expressed genes during HCC. Keywords: Genetic modification

2008-09-11 | GSE12723 | GEO

Wild-type versus HBx-transfected mive

2010-06-19 | E-GEOD-12723 | biostudies-arrayexpress

Effect of overexpressing of hepatitis B protein x (HBx) on the expression of circular RNAs (circRNAs) in HepG2 cells

Project description:CircRNA-sequencing in HBx-overexpressing (HBx-oe) and NC (negative control) HepG2 cells

2022-10-18 | GSE215232 | GEO

Differential gene expression profiling of MIHA cells transfected with C-terminal truncated HBx mutants

Project description:To compare the differentially expressed transcriptomes between MIHA cells transfected with empty vector control or different C-terminal truncated HBx mutants (14 or 35 amino acid carboxyl-terminal truncation - i.e. d14 and d35) mRNA profiles of MIHA cells stably overexpressing empty vector control or different C-terminal truncated HBx mutants (delta 14 and delta 35) were generated by PolyA mRNA sequencing using Illumina HiSeq 1500 platform

2016-08-02 | E-GEOD-71993 | biostudies-arrayexpress

Expression data from Hela cells stably expressing the wild-type HBx and the four mutant HBx

Project description:In large cohort studies, several HBx mutants were identified as independent risk factors for HCC risk.In order to study the carcinogenic mechanism between different mutants, HeLa cell lines expressing wild type and different mutated HBx proteins were constructed. We used microarrays to describe gene expression in HeLa cells stably expressing the wild-type HBx and the four mutant HBx, and to identify distinct classes of up-regulated genes.

2022-03-02 | GSE179126 | GEO

Differential gene expression profiling of MIHA cells transfected with C-terminal truncated HBx mutants

2016-08-02 | GSE71993 | GEO

Identification of substrates of the CRL4-HBx E3 ubiquitin ligase

Project description:Hepatitis B Virus (HBV) remains a major public health problem, and is a major cause liver cancer worldwide. HBV infection in vivo requires the function of the HBx protein, which facilitates expression of viral genes from the episomal genome by an unknown mechanism. Evidence suggests that HBx functions as a targeting subunit for the CRL4 E3 ubiquitin ligase, redirecting the complex to target and degrade an unknown host restriction factor. We used substrate trapping proteomics to search for ubiquitylation substrates of HBx. We used MLN4924 to block CRL activity and stabilize interactions between HBx and its substrates. We then performed APMS to identify bound proteins and potential substrates.

2016-09-29 | PXD004762 | Pride

HBX-HepG2 versus Control-HepG2

Project description:Mature microRNA expression profiling of HBx-expressing HepG2 cells versus Control cells under UV stress The mature microRNA expression profile of HBx-expressing HepG2 cells versus control cells in three independent biological repeated experiments. This study focuses only on human sequences. The complete data matrix is linked below as a supplementary file.

2010-05-16 | E-GEOD-15554 | biostudies-arrayexpress

miRNAs expression in HepG2-HBx and HepG2-URG11

Project description:Compare microRNAs expression when HepG2 cells stably expressing HBx protein with HepG2 control cells expressing baterial chloramphenicol acetyltransferase Compare microRNAs expression when HepG2 cells stably expressing URG11 protein with HepG2 control cells expressing baterial chloramphenicol acetyltransferase microRNA expression in Cells expressing HBx vs. Cell without HBx microRNA expression in Cells expressing URG11 vs. Cell without URG11

2011-11-23 | E-GEOD-33854 | biostudies-arrayexpress

CircRNAs in Arabidopsis thaliana across the lifespan

Project description:To explore the overall circRNAs involved in growth and development of Arabidopsis thaliana across the lifespan, we deeply sequenced samples of whole plants from different developmental stages (cotyledons emergence, rosette leavesï¹¥1 mm, rosette growth complete, first flower open, flourishing florescence, first silique shattered, senescence). The total RNA was purified by rRNA-depletion and linear RNA removal with RNAseR, and sequenced by the Illumina HiSeq2500 platform. We obtained 31 Gb raw data and identified 1217 circRNAs with expression quantity. We annotated these circRNAs and predicted their targeted microRNA. The circRNAs involved in growth and development of Arabidopsis thaliana across lifespan were identified and analyzed using the Illumina HiSeq2500 platform.

2016-09-01 | E-GEOD-77672 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data