Genomics

Dataset Information

0

NFIA determines the cis-effect of genetic variation on adipocyte browning in mice [ChIP-Seq]


ABSTRACT: Thermogenic brown and beige adipocytes counteract obesity by enhancing energy dissipation via uncoupling proein-1 (Ucp1). However, the effect of genetic variation on these cells, a major source of disease susceptibility, has been less well studied. Here we examined beige adipocytes from obesity-prone C57BL/6J (B6) and obesity-resistant 129X1/SvJ (129) mouse strains and identified a cis-regulatory variant rs47238345 that is responsible for differential Ucp1 expression. The alternative T allele of rs47238345 at the Ucp1 -12kb enhancer in 129 facilitates the allele-specific binding of nuclear factor I-A (NFIA) to mediate allele-specific enhancer-promoter interaction and Ucp1 transcription. We also identified Lim homeobox protein 8 (Lhx8), whose expression is higher in 129 than in B6, as a trans-acting regulator of Ucp1 in mice and humans. These results demonstrate the cis- and trans-acting effects of genetic variation on Ucp1 expression that underlie phenotypic diversity.

ORGANISM(S): Mus musculus

PROVIDER: GSE186904 | GEO | 2022/07/27

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2022-07-27 | GSE188260 | GEO
2022-07-27 | GSE186903 | GEO
2022-07-27 | GSE188261 | GEO
2017-10-28 | E-MTAB-4085 | biostudies-arrayexpress
2017-02-27 | GSE92606 | GEO
2017-02-27 | GSE91067 | GEO
2022-02-17 | PXD024717 | Pride
2024-07-30 | GSE184706 | GEO
2024-07-30 | GSE184687 | GEO
2024-07-30 | GSE270706 | GEO