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Chromatin Profiling by Directly Sequencing Small Quantities of Immunoprecipitated DNA

ABSTRACT: Chromatin structure and transcription factor localization can be assayed genome-wide by sequencing genomic DNA fractionated by protein occupancy or other properties. However, current technologies involve multiple steps that introduce bias and inefficiency. Here we apply a single-molecule approach to directly sequence chromatin immunoprecipitated DNA with minimal sample manipulation. This method is accurate, compatible with just 50 picograms of DNA and should thus facilitate charting chromatin maps from limited cell populations.

ORGANISM(S): Mus musculus

PROVIDER: GSE18699 | GEO | 2009/11/29

SECONDARY ACCESSION(S): PRJNA121485

REPOSITORIES: GEO

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Chromatin Profiling by Directly Sequencing Small Quantities of Immunoprecipitated DNA

Project description:Chromatin structure and transcription factor localization can be assayed genome-wide by sequencing genomic DNA fractionated by protein occupancy or other properties. However, current technologies involve multiple steps that introduce bias and inefficiency. Here we apply a single-molecule approach to directly sequence chromatin immunoprecipitated DNA with minimal sample manipulation. This method is accurate, compatible with just 50 picograms of DNA and should thus facilitate charting chromatin maps from limited cell populations. Application of a single-molecule approach to directly sequence chromatin immunoprecipitated DNA of the CTCF DNA binding protein.

2009-11-29 | E-GEOD-18699 | biostudies-arrayexpress

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