Transcriptomics,Genomics

Dataset Information

37

MicroRNA expression profiling in adult mouse liver following benzo(a)pyrene (BaP) treatment [Agilent miRNA array]


ABSTRACT: Lack of change in microRNA expression in adult mouse liver following treatment with benzo(a)pyrene (BaP), as detected using Agilent miRNA arrays. We have investigated the effect of exposure to 150 mg/kg benzo(a)pyrene (BaP) for 3 days on mRNA and miRNA expression levels in adult mouse liver. We used Agilent miRNA array platforms to assess effects of BaP exposure on miRNA expression levels. Our results indicate a distinct lack of effect of BaP of miRNA expression, despite widespread changes in mRNA levels. Keywords: Toxicology, miRNA Overall design: Adult male B6C3F1 mice were exposed to a daily dose of corn oil (vehicle control group) or 150 mg/kg BaP (treatment group) for 3 d (n=6 per group). Mice were sacrificed at 4 h or 24 h following the last dose and liver lobes were extracted and flash frozen. Agilent miRNA arrays were used to examine changes in miRNA transcript levels in random liver lobe sections.

INSTRUMENT(S): Agilent-019119 Mouse miRNA Microarray 1.0 G4472A

SUBMITTER: Andrew Williams 

PROVIDER: GSE18786 | GEO | 2010-10-18

SECONDARY ACCESSION(S): PRJNA123861

REPOSITORIES: GEO

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Publications

Lack of change in microRNA expression in adult mouse liver following treatment with benzo(a)pyrene despite robust mRNA transcriptional response.

Yauk Carole Lyn CL   Jackson Kelly K   Malowany Morie M   Williams Andrew A  

Mutation research 20100225 2


Benzo(a)pyrene (BaP) is a mutagenic and carcinogenic environmental contaminant. Metabolic activation of BaP is required for it to exert its mutagenic effects. Metabolism occurs via BaP interaction with the aryl hydrocarbon receptor (AHR) resulting in induction of phase 1 enzymes. Exposure to BaP is expected to cause differential regulation of AHR-responsive genes as well as pathways responding to DNA damage induced by its metabolites. MicroRNAs (miRNAs) are short non-coding molecules that contro  ...[more]

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