Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Comparison of diploid and transformed tetraploid MEF cells: gene expression and DNA methylation profiling

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Mus musculus

PROVIDER: GSE18815 | GEO | 2012/04/30

SECONDARY ACCESSION(S): PRJNA121237

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Comparison of diploid and transformed tetraploid MEF cells: gene expression and DNA methylation profiling

Project description:This SuperSeries is composed of the following subset Series: GSE18802: Comparison of gene expression profiles in diploid and transformed tetraploid MEF cells GSE18814: Comparison of DNA methylation profiles in diploid and transformed tetraploid MEF cells Refer to individual Series

2012-04-29 | E-GEOD-18815 | biostudies-arrayexpress

Comparison of DNA methylation profiles in diploid and transformed tetraploid MEF cells

2012-04-30 | GSE18814 | GEO

Comparison of DNA methylation profiles in diploid and transformed tetraploid MEF cells

Project description:We propose that tetraploidy induces epigentic changes including DNA methylation due to the abnormal chromatin in the cells. To test our hypothesis, we employed methylated CpG island recovery assay (MIRA) assisted microarray to determine the DNA methylation profiles in both diploid and tetraploid cells. First, we conducted a methylated-CpG island recovery assay (MIRA). Briefly, we enriched for methylated CpG islands with methylated DNA binding proteins, MBD2/MBD3L1. The pulled down DNA fragments containing CpG islands and input DNA fragments were amplified with real-time PCR. After labeling, they were hybridized to CpG island promoter array. Data were collected and analyzed.

2012-04-29 | E-GEOD-18814 | biostudies-arrayexpress

Comparison of gene expression profiles in diploid and transformed tetraploid MEF cells

2012-04-30 | GSE18802 | GEO

Comparison of gene expression profiles in diploid and transformed tetraploid MEF cells

Project description:We propose that abnormal DNA content in tetraploid cells can alter the gene expression profile, contributing to the cellular transformation. To test this hypothesis, we employ mRNA microarray to compare the gene expression profile in transformed tetraploid cells with that in the parent diploid cells. Keywords: gene expression profile determination Total RNA were extracted from diploid or tetraploid cells (n = 3 for each cell lines. mRNA microarray hybridization was conducted. Data were collected and analyzed.

2012-04-29 | E-GEOD-18802 | biostudies-arrayexpress

Characteristics of SP600125 Induced Tetraploid Cells in Comparison With Diploid and Tetraploid Cells of Fish.

Project description: Not available

| S-EPMC8685524 | biostudies-literature

Comparison of primary MEF vs HRasV12 + Twist transformed MEF

Project description:MEF cells were sequentially infected with H-RasV12 and Twist (Twist1 or Twist2) expression retroviral constructs. Gene expression profiles of the resulting transformed cell lines were compared to the gene expression profile of primary MEF cells

2008-07-11 | GSE11756 | GEO

Comparison of DNA methylation in human tissues

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2013-03-28 | E-GEOD-39777 | biostudies-arrayexpress

Genome-wide analysis and comparison of the DNA-binding one zinc finger gene family in diploid and tetraploid cotton (Gossypium).

Project description: Not available

| S-EPMC7323982 | biostudies-literature

Differential expression in response to water deficit in diploid leaves of sweet orange scion grafted alternatively on a diploid or auto-tetraploid Rangpur lime rootstock

Project description:This SuperSeries is composed of the SubSeries listed below.

2012-10-05 | GSE41311 | GEO