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Depletion of XRN1 from human cell lines

ABSTRACT: XRN1 is a cytoplasmic 5’-3’ exoribonuclease responsible for degradation of multiple types of RNA. Data obtained on yeast model described XRN1 function in degradation of messenger (m)RNA, Rapid transfer (t)RNA Decay (RTD) of hypomodified and unstable tRNA transcripts as well as maturation of 25S ribosomal (r)RNA and small nucleolar (sno)RNAs. Our group discovered previously that deletion of XRN1 gene results in stabilization of a group of long non-coding RNAs, which are often transcribed antisense to protein-coding genes and may regulate their expression on transcriptional level. However much less is known about human (h)XRN1 function. Data available from human cell lines confirm involvement of hXRN1 in degradation of mRNA, initiator tRNA methionine and some miRNA. The high sequence similarity of XRN1 between yeast and man suggests that also its function should be well conserved for hXRN1. To determine the cytoplasmic transcripts dependent on XRN1 activity we have constructed an auxin-degron system with inducible depletion of XRN1 protein in cell line, combined with subcellular fractionation followed by high-throughput sequencing. The raw data and counts for annotated and non-annotated transcripts resulting from this experiment are provided.

ORGANISM(S): Homo sapiens

PROVIDER: GSE188282 | GEO | 2026/06/02

REPOSITORIES: GEO

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