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Determining the Translational Potential of Human Pericardial Fluid to Improve Clinical Outcomes in Cardiac Surgery

ABSTRACT: The pericardial space, which contains pericardial fluid (PF), maintains homeostatic conditions that facilitates optimal heart function. PF can carry biomarkers for different cardiac diseases. PF can also play a central role in delivering targeted therapeutics. The loss of PF during surgery perturbs the equilibrium of this environment, contributing to postsurgical complications, including postoperative pericardial adhesion formation (PPAF) and postoperative atrial fibrillation (POAF), both of which involve immune-mediated pathways. We have yet to fully understand the exact pathophysiology of these adverse events. This study aimed to establish the immune profile of human PF, determine whether specific markers can drive inflammatory processes, such as fibrosis, and elucidate the underlying mechanisms that can result in such phenomena.

ORGANISM(S): Homo sapiens

PROVIDER: GSE188890 | GEO | 2026/07/09

REPOSITORIES: GEO

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Pericardial fluid exosome miRNA profiling

Project description:Pericardial sac surrounding the heart contains pericardial fluid (PF), which is rich in exosomes. PF exosomes increase angiogenesis in hypoxic endothelial cells and in animal model of hindlimb ischemia by passing the proangiogenic miRNAs to recipient cells. However, under pathological conditions such as diabetes, exosome cargo composition changes and harmful miRNAs can be transferred to the recipient cells and induce more deleterious effects in target tissues. In order to check cargo composition of different PF exosomes, we used PF exosomes from non-diabetic aortic valve replacement (AVR), mitral valve replacement (MVR), coronary artery bypass grafting (CABG) patients and CABG patients with diabetes.

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Project description:Pericardial fluid (PF) helps maintain homeostatic conditions that facilitate optimal heart function. We have yet to understand how cardiovascular pathologies affect the profile of PF. We hypothesized that human PF contains immune markers that can be affected by different pathologies and contribute to inflammatory-mediated processes, such as fibrosis. PF was collected from patients undergoing cardiac surgery. Multiplex analysis was performed to determine the composition of inflammatory markers and soluble mediators. A gel contraction assay was used to investigate the fibrotic potential of PF. RNA-sequencing was used to elucidate the mechanisms driving the pro-fibrotic properties of PF. With the results of these multiple approaches we show that the acellular component of PF has pro-fibrotic characteristics and suggest putative mechanisms that can explain such observations.

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2013-07-09 | PXD000194 | Pride

Hematopoietic somatic mosaicism is associated with an increased risk of postoperative atrial fibrillation

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