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Transcriptional regulator HDP1 regulates expansion of the inner membrane complex during early sexual differentiation of malaria parasites

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Plasmodium falciparum

PROVIDER: GSE189197 | GEO | 2021/11/21

REPOSITORIES: GEO

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The transcriptional regulator HDP1 controls expansion of the inner membrane complex during early sexual differentiation of malaria parasites.

Project description: Not available

| S-EPMC8852293 | biostudies-literature

Transcriptional regulator HDP1 regulates expansion of the inner membrane complex during early sexual differentiation of malaria parasites [ChIP-Seq]

Project description:In this study, we identify HDP1, a previously uncharacterized DNA-binding protein first expressed during early sexual differentiation. The development of HDP1-deficient gametocytes arrests at the Stage I to Stage II transition and ends in loss of viability. Analysis of gene expression and HDP1-binding shows that this protein functions as a positive transcriptional regulator of genes essential for gametocyte development, including genes that are critical for the expansion of the inner membrane complex (IMC) that gives P. falciparum gametocytes their characteristic shape.

2021-11-21 | GSE189151 | GEO

Transcriptional regulator HDP1 regulates expansion of the inner membrane complex during early sexual differentiation of malaria parasites [RNA-Seq]

2021-11-21 | GSE189196 | GEO

Transcriptional regulator HDP1 regulates expansion of the inner membrane complex during early sexual differentiation of malaria parasites

Project description:Transcriptional regulator HDP1 regulates expansion of the inner membrane complex during early sexual differentiation of malaria parasites

| PRJNA781928 | ENA

Transcriptional regulator HDP1 regulates expansion of the inner membrane complex during early sexual differentiation of malaria parasites [ChIP-Seq]

Project description:Transcriptional regulator HDP1 regulates expansion of the inner membrane complex during early sexual differentiation of malaria parasites [ChIP-Seq]

| PRJNA781649 | ENA

Transcriptional regulator HDP1 regulates expansion of the inner membrane complex during early sexual differentiation of malaria parasites [RNA-Seq]

Project description:Transcriptional regulator HDP1 regulates expansion of the inner membrane complex during early sexual differentiation of malaria parasites [RNA-Seq]

| PRJNA781941 | ENA

Disrupting assembly of the inner membrane complex blocks Plasmodium falciparum sexual stage development.

Project description: Not available

| S-EPMC5646874 | biostudies-literature

An exon DNA element modulates heterochromatin spreading in the master regulator for sexual commitment in malaria parasites

Project description:Heterochromatin is essential in all eukaryotic systems to maintain genome integrity, long-term gene repression and to help chromosome segregation during mitosis. However, heterochromatin regions must be restricted by boundary elements to avoid its spreading over actively transcribed loci. In Plasmodium falciparum, facultative heterochromatin is important to regulate parasite virulence, antigenic variation and transmission, but the underlying molecular mechanisms that regulate repressive regions remain unknown. To investigate this topic, we chose the ap2-g gene, that forms a strictly delimited heterochromatin island of 10 kb with no adjacent repressive chromatin. Using electrophoretic motility shift assay (EMSA) we identified an ap2-g exon element at the 3? end that shifts in the presence of nuclear extracts. Upon replacement of this region by a gfp gene, we observed a shift in the heterochromatin boundary, resulting in HP1 to spread over ~2 additional kb downstream. We used this DNA element to affinity purify candidate proteins followed by liquid-chromatography tandem mass-spectrometry (LC-MS/MS). The identified complexes are enriched in RNA-binding proteins, pointing to a potential role of RNA in the regulation of the ap2-g heterochromatin 3? boundary. Our results provide insight into the unexplored topic of heterochromatin boundary biology in P. falciparum and identifies a DNA element in the master regulator of sexual commitment that modulates heterochromatin spreading.

2020-02-18 | GSE145378 | GEO

Regulation of sexual differentiation is linked to invasion in malaria parasites

Project description:This SuperSeries is composed of the SubSeries listed below.

2019-02-08 | GSE125566 | GEO

Inner membrane complex proteomics reveals a palmitoylation cascade regulating intraerythrocytic development of malaria parasite

Project description:Malaria is caused by infection of the erythrocytes by the parasites Plasmodium. Inside the erythrocytes, the parasites grow and multiply via schizogony, an unconventional cell division mode. The Inner Membrane Complex (IMC), an organelle located beneath the plasma membrane of the parasites, is essential for schizogony, serving as the platform for protein anchorage. However, the protein compositions of IMC and their localization determinants remain unclear. Here we used biotin ligase (TurboID)-based proximity labelling and quantitative mass spectrometry to compile the proteome of the schizont IMC of rodent malaria parasite Plasmodium yoelii. In total, the IMC proteome consisting of 488 proteins were identified. 19 of the 22 selected candidates were confirmed to localize in the IMC, indicating good reliability. In light of the existing palmitome of Plasmodium falciparum, 136 proteins of the P. yoelii IMC proteome are potentially palmitoylated. We further identified DHHC2 the major resident palmitoyl-acyl-transferase of the IMC. Conditional depletion of DHHC2 led to defective schizont segmentation and growth arrest in vitro and in vivo. DHHC2 was found to palmitoylate two critical IMC proteins CDPK1 and GAP45 for their IMC localization. In summary, this study reports a comprehensive inventory of P. yoelii IMC proteins and demonstrates a central role of DHHC2 governing IMC localization of proteins during the schizont development.

2022-07-01 | PXD028193 | iProX

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