YciR, a specific 3′-phosphodiesterases, plays a role in the pathogenesis of Uropathogenic Escherichia coli CFT073

ABSTRACT: Uropathogenic Escherichia coli are a prevailing causation of urinary tract infections infections and characterized by recurrence and resistance to antibiotics due to their misuse, causing a large economic burden to individuals and countries. An early determinant of the pathogenicity of UPEC is its ability to form intracellular bacterial communities in the cytoplasm of bladder epithelial cells after its invasion for evading immune response. Cyclic dimeric guanosine monophosphate (c-di-GMP) is a recently high frequency discussed small molecule from bacterial to trigger the innate immune response form the host while YciR has been demonstrated that had an ability to degrade the level of c-di-GMP. There is no definite conclusion in the contribution of YciR and the level of c-di-GMP in the pathogenesis of UPEC. In this study, we used transcriptomic techniques approach to analyze the gene expression profiles of UPEC at the stage of bacterial survival in bladder epithelial cells and we screened yciR gene that may be associated with virulence that was upregulated in the transcriptome by differential gene analysis. In the absence of yciR, there is a Phosphorylation of NF-kappaB analyzed by Western Blot which results in decreased bacterial colonization in vitro and a reducing in mice bladder. In this study, we identified for the first time that YciR as a virulence factor in the pathogenesis of UPEC and discovered that UPEC manipulate YciR to facilitate colonization in vitro and vivo causing acute bladder infection. In addition, we also found that hupB regulated the expression of yciR to mediate the Phosphorylation of NF-kappaB These findings further increase the understanding of the pathogenesis of UPEC and provide a theoretical basis for further studies.

ORGANISM(S): Escherichia coli CFT073

PROVIDER: GSE189294 | GEO | 2021/11/24

REPOSITORIES: GEO