Transcriptomics

Dataset Information

0

Distinct translatome changes in specific neural populations precede electroencephalographic changes in prion-infected mice


ABSTRACT: Selective vulnerability is an enigmatic feature of neurodegenerative diseases (NDs), whereby a widely expressed protein causes lesions in specific cell types and brain regions. Using the RiboTag method in mice, translational responses of five neural subtypes to acquired prion disease (PrD) were measured. Pre-onset and disease onset timepoints were chosen based on longitudinal electroencephalography (EEG) that revealed a gradual increase in theta power between 10- and 18-weeks after prion injection, resembling a clinical feature of human PrD. At disease onset, marked by significantly increased theta power and histopathological lesions, mice had pronounced translatome changes in all five cell types despite appearing normal. Remarkably, at a pre-onset stage, prior to EEG and neuropathological changes, we found that 1) translatomes of astrocytes indicated reduced synthesis of ribosomal and mitochondrial components, 2) glutamatergic neurons showed increased expression of cytoskeletal genes, and 3) GABAergic neurons revealed reduced expression of circadian rhythm genes. These data demonstrate that early translatome responses to neurodegeneration emerge prior to conventional markers of disease and are cell type-specific. Therapeutic strategies may need to target multiple pathways in specific populations of cells, early in disease.

ORGANISM(S): Mus musculus

PROVIDER: GSE189527 | GEO | 2022/06/13

REPOSITORIES: GEO

Similar Datasets

2020-06-21 | GSE137264 | GEO
2022-10-07 | GSE196491 | GEO
2023-01-01 | GSE207251 | GEO
2017-02-13 | GSE90485 | GEO
2014-03-11 | GSE55495 | GEO
2014-03-11 | GSE55494 | GEO
2024-05-03 | GSE255987 | GEO
2012-11-09 | E-GEOD-34530 | biostudies-arrayexpress
2023-01-01 | GSE144640 | GEO
2017-05-15 | GSE90977 | GEO