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Early-life thermal stress mediates long-term alterations in hypothalamic microglia [RNA-Seq]

ABSTRACT: We identified the microglial population in the chick anterior hypothalamus (AH) and showed that early-life thermal stress (on day 3 post-hatch) influences the inflammatory process in the AH, altering microglial transcriptomic signature in response to LPS treatment (on day 10 post-hatch).

ORGANISM(S): Gallus gallus

PROVIDER: GSE189566 | GEO | 2021/12/21

REPOSITORIES: GEO

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Early-life thermal stress mediates long-term alterations in hypothalamic microglia [RRBS]

Project description:We identified the microglial population in the chick anterior hypothalamus (AH) and showed that early-life thermal stress (on day 3 post-hatch) influences the inflammatory process in the AH. We described alterations in genome-wide CpG methylation profile of hypothalamic microglia between heat-conditioned and non-conditioned 10-day-old chicks prior LPS challenge. We suppose that changes in CpG DNA methylation might be involved in reprogramming microglial function.

2021-12-21 | GSE189564 | GEO

Deep Sequencing Analysis of miRNAs Expression in leg muscle of Xinghua Chicken

Project description:Purpose:We have used RNA-seq to examine of differentially expressed miRNAs in chicken leg muscle of three different development stages (11 embryo ages, 16 embryo ages, and 1 day old post hatch chick).The aims of this study are characterization of miRNAs differentially expressed in different developmental stage of chicken embryo, using RNA sequence sample. Methods: On this study we used two embryonic stage and one post hatch chick leg muscle of Xinghua chicken breed. Total RNA from E11 day embryo, E16 day embryo and 1 day post hatch chick was isolated by TRIzol following the manufacturer’s protocol (Invitrogen, CA, USA). Each stages were designed two samples, and the total samples were six (three group × two sample/group) and RNA samples of six individuals were pooled with equal amounts, and then were subjected to Illumina deep sequencing. Results: After eliminating adaptor and low-quality reads, a total of 5,302,700, 6,556,747, 5,359,793, 4,213,112, 7,112,885 and 7,469,939 clean reads were obtained in group E11 (E11.1-E11.2), group E16 (E16.1-E16.2) and group P1 (P1.1–P1.2) libraries, respectively. The clean reads were aligned to the chicken genome databases, miRBase, Rfam, RepBase and mRNA. Conclusions:To assess miRNA expression during chicken embryo skeletal muscle development, we sequenced and analyzed leg muscle at 11 day embryo age, 16 day embryo age, and 1 days post hatch.

2017-06-22 | GSE100321 | GEO

Coral thermal tolerance: tuning gene expression to resist thermal stress

Project description:Thermal history plays a role in the response of corals to subsequent heat stress. Prior heat stress can have a profound impact on later thermal tolerance, but the mechanism for this plasticity is not clear. The understanding of gene expression changes behind physiological acclimatization is critical in forecasts of coral health in impending climate change scenarios. Acropora millepora fragments were preconditioned to sublethal bleaching threshold stress for a period of 10 days; this prestress conferred bleaching resistance in subsequent thermal challenge, in which non-preconditioned coral bleached. Using microarrays, we analyze the transcriptomes of the coral host, comparing the bleaching-resistant preconditioned treatment to non-preconditioned and control treatments. This experiment compared host gene expression of Acropora millepora across control, non-preconditioned, and preconditioned treatments. Fragments were sampled prior to preconditioning (Day 4), following 10 days of thermal preconditioning (Day 20), and after two (Day 23), four (Day 25), and eight days (Day 29) of 31M-BM-0C thermal challenge. The analysis implements 45 arrays, representing 5 sampling points of three treatments (n=3).

2012-11-29 | E-GEOD-41435 | biostudies-arrayexpress

Gene expression in three different chicken lines after Salmonella infection early in life

Project description:Transcriptional profiling of jejunum infected with Salmonella in three different chicken lines early in life. Samples were taken at 8, 24 and 48 hours post infection. Salmonella was orally ingested at day zero (hatch).

2012-01-29 | GSE27069 | GEO

Early-life thermal stress mediates long-term alterations in hypothalamic microglia

Project description:Early-life thermal stress mediates long-term alterations in hypothalamic microglia

| PRJNA783611 | ENA

Thermal transcriptome

Project description:Thermal transcriptome

| PRJNA772171 | ENA

Early-life thermal stress mediates long-term alterations in hypothalamic microglia [RRBS]

Project description:Early-life thermal stress mediates long-term alterations in hypothalamic microglia [RRBS]

| PRJNA783616 | ENA

Gene expression in three different chicken lines after Salmonella infection early in life

2012-01-29 | E-GEOD-27069 | biostudies-arrayexpress