Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Identification and characterization of BEND2 as a novel and key regulator of meiosis during mouse spermatogenesis [ATAC_seq]

ABSTRACT: To describe the changes of chromatin accessibility in Bend2 knockout mice, we conducted ATAC-seq analyses by using zygotene spermatocytes from Bend2 WT and KO mice. We found the chromatin state were opened in the proximal promoter of Bend2 up-regulated genes.

ORGANISM(S): Mus musculus

PROVIDER: GSE189597 | GEO | 2022/06/01

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Identification and characterization of BEND2 as a novel and key regulator of meiosis during mouse spermatogenesis [Histone_CUTRUN]

Project description:To describe the changes of histone modifications in Bend2 knockout mice, we conducted CUT&RUN analyses by using zygotene spermatocytes from Bend2 WT and KO mice with H3K4me3 and H3K27me3 antibodies. We found the enrichment of these histone modifications in the proximal promoter of Bend2 down-regulated genes were disrupted in Bend2 KO mice.

2022-06-01 | GSE189596 | GEO

Identification and characterization of BEND2 as a novel and key regulator of meiosis during mouse spermatogenesis [RNA-seq]

Project description:To describe the changes of transcriptome in Bend2 knockout mice, we conducted RNA-seq analyses by using leptotene and zygotene spermatocytes from Bend2 WT and KO mice. We found BEND2 contributes to shutting down the mitotic program and to activating or enhancing the meiotic and post-meiotic program of spermatogenic cells.

2022-06-01 | GSE189598 | GEO

Identification and characterization of BEND2 as a novel and key regulator of meiosis during mouse spermatogenesis [RNA-Seq II]

Project description:To describe the changes of transcriptome in Bend2 knockout mice, we conducted RNA-seq analyses by using spermatocytes from d12 (12-days after birth) Bend2 WT and KO mice. We found BEND2 contributed to inhibiting the expression of genes involved in spermatogonia differentiation and meiosis initiation.

2022-06-01 | GSE196004 | GEO

Identification and characterization of BEND2 as a novel and key regulator of meiosis during mouse spermatogenesis [BEND2_ChIPseq]

Project description:To characterize BEND2-binding sites on the genome, we conducted ChIP-seq analyses by using testicular lysates from FLAG-BEND2 KI mice. We found BEND2 preferentially binds to simple sequence repeats.

2022-06-01 | GSE189594 | GEO

Screening of genes involved in chromosome segregation in meiosis I

Project description:Prophase I of male meiosis involves dynamic chromosome segregation processes during early spermatogenesis, including synapsis, meiotic recombination, and cohesion. Genetic defects in genes participating in these processes consistently cause reproduction failure in mice. To identify candidate genes responsible for infertility in humans, we performed expression profiling of mouse spermatogenic cells undergoing meiotic prophase I. Cell fractions enriched in spermatogonia, leptotene/zygotene spermatocytes, or pachytene spermatocytes were separately isolated from mouse testes for RNA extraction. To minimize the contamination of other cell types, we fractionated the testicular cells undergoing the first round of spermatogenesis using Percoll gradient procedure. The cell fractions were characterized by morphological analysis by phase contrast microscopy and Nomarski interference microscopy, and then expression of cell lineage- and spermatogenesis stage-specific genes were examined by RT-PCR. The most enriched fractions for spermatogonia (fraction2 from day8), leptotene/zygotene spermatocytes (fraction5 from day12), and pachytene spermatocytes (fraction8 from day15) were subjected to hybridization on Affymetrix microarrays.

2010-03-31 | E-GEOD-19532 | biostudies-arrayexpress

Multiple epigenetic mechanisms and the piRNA pathway enforce LINE1 silencing during adult spermatogenesis

Project description:The Piwi protein Mili and the Piwi-interacting RNA (piRNA) pathway are required to establish L1 DNA methylation during epigenetic reprogramming that accompanies male germ cell development. Although Mili is expressed throughout most of adult spermatogenesis, its function therein remains unknown. We find that Mili displays a hitherto unappreciated dynamic expression profile during spermatogenesis, expressed in the mitotic spermatogonia but absent at the early meiotic stages only to reappear in late zygotene spermatocytes. Here we show using conditional mutagenesis that Mili's endonuclease activity post-transcriptionally cleaves L1 transcripts commencing upon its reappearance in late zygotene spermatocytes even in the presence of normal L1 DNA methylation

2013-07-03 | E-MTAB-1557 | biostudies-arrayexpress

Gene and retrotransposon expression analysis for WT, Pld6 KO, and Dnmt3l KO male germ cells during spermatogenesis

Project description:mRNA sequencing analysis of P0 testes and FACS-purified leptotene/zygotene (L/Z) spermatocytes

2017-07-20 | GSE70889 | GEO

Identification and characterization of BEND2 as a novel and key regulator of meiosis during mouse spermatogenesis

Project description:Identification and characterization of BEND2 as a novel and key regulator of meiosis during mouse spermatogenesis

| PRJNA783812 | ENA

INO80 chromatin binding profile in mouse zygotene-spermatocytes

Project description:INO80 chromatin binding profile in mouse zygotene-spermatocytes

| PRJNA787614 | ENA

Small RNA and transcriptome profiling of mice deficient in either germline Dgcr8 or Dicer

Project description:We used Illumina Small RNA and RNA-Seq kits to prepare both small RNA and RNA-Seq libraries from total RNA isolated from either leptotenze/zygotene or pachytene spermatocytes purified from either Dgcr8 or Dicer germline conditional knockout mice. Conditional knockout mice were generated by using a Ddx4 promoter to drive cre excision of either Dgcr8 or Dicer at embryonic day 18. Mixed leptotene/zygotene or pachytene spermatocytes were then isolated from the testis of adult conditional knockout mice, along with paired WT littermates as a control. RNA was isolated from these spermatocytes using Trizol. Small RNA or RNA-Seq libraries were then prepped using Illumina's sequencing library preparation kits.

2015-05-06 | E-GEOD-63166 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data