Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA sequencing analysis of the hippocampus reveals transcriptomic signatures of PTZ-kindled seizure rats and upon treatment of novel anti-convulsant chemical Q808

ABSTRACT: The present study aimed to use RNA sequencing (RNA-seq) to reveal the gene transcriptomic profile of chronic PTZ-kindled seizure rats and the difference of the PTZ model rat before and after treatment with Q808.

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE189785 | GEO | 2021/12/01

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Hippocampus RNA-sequencing of Q808 against PTZ-induced seizure model

Project description:The present study aimed to use RNA sequencing to reveal the gene transcriptomic profile of PTZ-induced seizure rats and the difference of the PTZ model rat before and after treatment with Q808.

2022-03-01 | GSE193580 | GEO

RNA sequencing analysis of the hippocampus reveals transcriptomic signatures of PTZ-kindled seizure rats and upon treatment of novel anti-convulsant chemical Q808

Project description:RNA sequencing analysis of the hippocampus reveals transcriptomic signatures of PTZ-kindled seizure rats and upon treatment of novel anti-convulsant chemical Q808

| PRJNA784521 | ENA

Hippocampus RNA-sequencing of Q808 against PTZ-induced seizure model

Project description:Hippocampus RNA-sequencing of Q808 against PTZ-induced seizure model

| PRJNA796655 | ENA

Cortical and hippocampal gene expression after transcranial focal stimulation and a single seizure in rats

Project description:Transcranial Electrical Stimulation has therapeutic effects on different diseases, including epilepsy. Particularly, Transcranial Focal Stimulation (TFS) has anticonvulsive and antiepileptogenic effects. Nevertheless, the mechanisms and therapeutic targets that induced these effects remain ellusive. We aimed to screen the genetic expression in the cerebral cortex and the hippocampus of rats after a single seizure and TFS to identify potential targets.

2024-03-30 | GSE199183 | GEO

Leukocyte gene expression predicts human temporal lobe epilepsy seizure frequency

Project description:This study was performed to test the hypothesis that systemic leukocyte gene expression has prognostic value differentiating low from high seizure frequency refractory temporal lobe epilepsy (TLE). A consecutive series of sixteen patients with refractory temporal lobe epilepsy was studied. Based on a median baseline seizure frequency of 2.0 seizures per month, low versus high seizure frequency was defined as < 2 seizures/month and > 2 seizures/month, respectively.

2022-11-15 | GSE217726 | GEO

Transcriptome analysis of CA1 rat hippocampus 1 week after kainate seizure at P7

Project description:Seizures that occur during early development are associated with adverse neurodevelopmental outcomes. Causation and mechanisms are currently under investigation. Induction of an early life seizure by kainic acid (KA) in immature rats on post-natal day (P) 7 results in behavioral changes in the adult rat that reflect social and intellectual deficits without overt cellular damage. Our previous work also demonstrated increased expression of CA1 hippocampal long-term potentiation (LTP) and reduced desensitization of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid-type ionotropic glutamate receptors (AMPA-R) one week following a kainic acid induced seizure (KA-ELS). Here we used RNA sequencing (RNAseq) of mRNA from dorsal hippocampal CA1 to probe changes in mRNA levels one week following KA-ELS as a means to investigate the mechanisms for these functional changes. Ingenuity pathway analysis (IPA) confirmed our previous results by predicting an up-regulation of the synaptic LTP pathway. Differential gene expression results revealed significant differences in 7 gene isoforms. Additional assessments included AMPA-R splice variants and adenosine deaminase acting on RNA 2 (ADAR2) editing sites as a means to determine the mechanism for reduced AMPA-R desensitization. Splice variant analysis demonstrated that KA-ELS result in a small, but significant decrease in the "flop" isoform of Gria3, and editing site analysis revealed significant changes in the editing of a kainate receptor subunit, Grik2, and a serotonin receptor, Htr2c. While these specific changes may not account for altered AMPA-R desensitization, the differences indicate that KA-ELS alters gene expression in the hippocampal CA1 one week after the insult.

2020-04-17 | GSE136913 | GEO

microRNAs profiling in hippocampal cells of mouse with Sound-induced Seizure

Project description:After an electrically induced seizure, mice (NMRI) received electrical stimulation (current intensity 44 mA, 0.2 ms monopolar pulses at 6 Hz frequency for 3 s). RNA was extracted from the hippocampi at 0, 3, 6, 24 and 72 h after the seizure. microRNA expressions were measured via microarray technology using Exiqon's miCURY™ LNA Arrays. Time-course design, treated cells at 0, 3, 6, 24, and 72 h, with 7 replicate each

2014-01-01 | E-GEOD-51840 | biostudies-arrayexpress

microRNAs profiling in hippocampal cells of mouse with Sound-induced Seizure

2014-01-01 | GSE51840 | GEO

Profiling the transcriptome of excitatory neuronal nuclei during pilocarpine-induced seizure

Project description:The initial seizure-induced events in neuronal nuclei can lead to long-term changes in gene expression and cellular responses that likely contribute toward epileptogenesis. Using a transgenic mouse model to specifically isolate excitatory neuronal nuclei, we profiled the seizure-induced nuclear transcriptome by RNA-seq, as well as proteome via tandem mass tag mass spectrometry (available at ProteomeXchange via PRIDE; Accession ID: PXD030637). In the transcriptome, we observed robust induction of genes involved in multiple processes, including MAPK signalling, and downregulation of zinc finger genes harbouring Krüppel-associated box (KRAB) domains, which were strongly enriched for binding sites of the Thap11/Ronin transcriptional repressor. Additionally, we characterised gene expression in introns, which provided further insights into seizure-driven transcriptional regulation and dynamics, including repression of transcription mediated by zinc finger transcription factors. Our results provide a detailed snapshot of nuclear events induced by seizure activity and demonstrate a robust method for cell-type-specific nuclear profiling that can be applied to other cell types and models.

2023-03-31 | GSE205454 | GEO

Transcriptome analysis of CA1 rat hippocampus 1 week after kainate seizure at P7

Project description:Transcriptome analysis of CA1 rat hippocampus 1 week after kainate seizure at P7

| PRJNA564028 | ENA