MeRIP-seq to explore the targets of METTL3 in pancreatic cancer

ABSTRACT: We performed MeRIP-seq of METTL3-knockdown (KD) MIAPaCa-2 and control cells to detect METTL3-methylated genes and extracted genes whose methylation was lost in METTL3-KD cells compared with control cells. Next, we performed mRNA expression analysis using dates of input samples at MeRIP-seq. Genes whose methylation peaks were abolished by METTL3-KD and whose expression changes were observed were analyzed as candidates for METTL3-regulated genes.

ORGANISM(S): Homo sapiens

PROVIDER: GSE190078 | GEO | 2021/12/13

REPOSITORIES: GEO