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Transcriptome analysis after GPX4 knockdown in primary mouse chondrocyte（mcc）

ABSTRACT: Osteoarthritis (OA) is the most common joint disease and is the leading cause of chronic disability among older people. Chondrocyte death was involved in OA pathogenesis. Ferroptosis is an iron-dependent cell death associated with peroxidation of lipids. Expression of GPX4 in the OA cartilage from OA patients were significantly lower than normal cartilage.In order to analyze the mechanism of GPX4, we conducted RNA-sequencing in mouse chondrocytes with or without GPX4 knockdown.Our results showed that Gpx4 downregulation could increase the sensitivity of chondrocytes to oxidative stress and aggravate ECM degradation in chondrocytes.

ORGANISM(S): Mus musculus

PROVIDER: GSE190184 | GEO | 2022/02/18

REPOSITORIES: GEO

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Autophagy of OTUD5 Destabilizes GPX4 to Confer Ferroptosis-Dependent Kidney Injury

Project description:Ferroptosis is an iron-dependent programmed cell death associated with severe kidney diseases, linked to decreased glutathione peroxidase 4 (GPX4). However, the spatial distribution of renal GPX4-mediated ferroptosis and the molecular events causing GPX4 reduction during ischemia-reperfusion (I/R) remain largely unknown. Using spatial transcriptomics, we identify that GPX4 is situated at the interface of the inner cortex and outer medulla, a hyperactive ferroptosis site post-I/R injury. We show that OTU deubiquitinase 5 (OTUD5) is a GPX4-binding protein that confers ferroptosis resistance by stabilizing GPX4. During I/R, ferroptosis is induced by mTORC1-mediated autophagy, causing OTUD5 degradation and subsequent GPX4 decay. Functionally, OTUD5 deletion intensifies renal tubular cell ferroptosis and exacerbates acute kidney injury, while AAV-mediated OTUD5 delivery mitigates ferroptosis and promotes renal function recovery from I/R injury. In this work, our study highlights a new autophagy-dependent ferroptosis module: hypoxia/ischemia-induced OTUD5 autophagy triggers GPX4 degradation, offering a potential therapeutic avenue for I/R-related kidney diseases.

2023-11-29 | GSE244330 | GEO

Gpx4 prevents Treg-cell lipid peroxidation and ferroptosis in promoting immune homeostasis and tumor immune escape

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2023-06-20 | PXD043133 | Pride

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2012-12-06 | E-GEOD-34215 | biostudies-arrayexpress

Transcriptome analysis after GPX4 knockdown in primary mouse chondrocyte（mcc）

Project description:Transcriptome analysis after GPX4 knockdown in primary mouse chondrocyte（mcc）

| PRJNA786223 | ENA

RNA-seq of human pancreatic cancer cell line PANC-1 treated with RSL-3, N6F11, Fin56 and Erastin against untreated controls

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2023-10-18 | E-MTAB-12241 | biostudies-arrayexpress

RNA-sequencing and Exome-sequencing analysis of ferroptosis-resistant 786-O GPX4-null carcinoma cells following in vivo selection

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