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Identification of Smad2 and Smad3 specific binding sites in a breast cancer cell line model

ABSTRACT: We identify Smad2 and Smad3 specific binding site in a breast cancer breast cell model MDA-MB-231 using LAP-tag GFP fusion proteins under the control of endogenous regulatory elements. Stable cell lines were generated via transfection with a BAC followed by antibiotics selection. We show that LAP-fused SMad2/3 can be used to recapitulate native TGFB signaling. Smad2/Smad3 specific binding sites were identified by ChIP-seq against GFP before and after TGFB treatment. Genomic DNA was sequenced via single end sequencing on Illumina HiSeq

ORGANISM(S): Homo sapiens

PROVIDER: GSE190237 | GEO | 2021/12/08

REPOSITORIES: GEO

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Smad2 and Smad3 have differential sensitivity in relaying TGF b signaling and inversely regulate early lineage specification

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| PRJNA786492 | ENA