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Circular RNA expression profile and its potential regulative role in Chronic total occlusion

ABSTRACT: Objectives: CircRNAs, a novel class of noncoding RNAs, have been reported in many diseases. However, their role in coronary CTO remains unclear. Here, we aimed to determine the circRNA expression profile in coronary CTO patients and further construct a circRNA–miRNA network. It provides a new way to study the pathogenesis of coronary CTO. Methods: Plasma circRNA expression profiles were screened by circRNA microarrays. The CircRNA–miRNA network was established to predict the circRNA targets. Gene ontology (GO) analysis and KEGG, Reactome pathway analysis were applied for further enrichment of data. Finally, quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed to confirm these results. Results: We collected the peripheral blood of 4 adult patients with coronary CTO and 4 adult patients with normal coronary arteries, and found 11,562 circRNAs by sequencing. 652 circRNAs were significantly differentially expressed, of which 196 were significantly up-regulated and 456 were significantly down-regulated. Through bioinformatics analysis, we analyzed and predicted the potential biological functions of circRNAs. Further studies found that differently expressed circRNAs are mainly involved in the occurrence and development of coronary CTO by participating in inflammatory response, angiogenesis, calcification in lesions, regulation of lipid metabolism, insulin secretion and other pathways. Finally, the over-expression of hsa_circ_0045503, hsa_circ_0125975 and under-expression of hsa_circ_0102054 were further validated by qRT-PCR. Hsa-circ-0125975 exhibited significant up-regulation in patients with coronary CTO, which was in line with the sequencing data. Conclusions: The results of this study may provide a new biomarker and therapeutic target for the diagnosis and treatment of coronary CTO.

ORGANISM(S): Homo sapiens

PROVIDER: GSE190251 | GEO | 2025/10/05

REPOSITORIES: GEO

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Project description:Background: Of Hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC), 85%-90% of cases develop from liver cirrhosis, and circular RNAs (circRNAs) have important roles in this process; however, differences in serum circRNA expression profiles between patients with HBV-related cirrhosis and those with HCC have not been studied. Methods: Serum RNA was extracted from patients with HCC and cirrhosis (n = 5 per group) and used for microarray analysis of circRNA expression profiles. Bioinformatics analyses, including clustering, differential expression, and construction of a ceRNA network, were performed. Quantitative real-time reverse transcription PCR validation analysis was conducted using samples from patients with HBV-related cirrhosis (n = 88) and HCC (n = 73). Further, statistical analyses were used to analyze the potential function and value of selected circRNAs with expression differing between the HBV-related cirrhosis and HCC groups. Results: Cluster analysis revealed 8 up-regulated and 80 down-regulated circRNAs. Further, qRT-PCR analysis showed that circRNA_0000367 expression was consistent with that detected by microarray experiments, with significantly lower levels in patients with HBV-related HCC than those with HBV-related cirrhosis. CircRNA_0000367 expression levels were also significantly lower in patients with drug-resistant HBV and those with HBV-related cirrhosis with Model for End-Stage Liver Disease score < 10. Further, circRNA_00000367 expression levels were lower in patients with HBV-related cirrhosis who progressed to HCC. Analysis of the lncRNA-miRNA-mRNA ceRNA network identified 39 miRNAs and 24 mRNAs involved in circRNA_00000367 networks; these target genes were involved in various biological processes and signaling pathways. Conclusion: Serum cicrRNA_0000367 is a potential HCC biomarker in patients with HBV-related cirrhosis, where down-regulation of cicrRNA_0000367 in HBV-related cirrhosis may be associated with progression to HCC.

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Circular RNA expression profile and its potential regulative role in Chronic total occlusion

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