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Translocated Legionella pneumophila small RNAs mimic eukaryotic miRNAs to dampen the host immune response [THP-1]

ABSTRACT: Extracellular vesicles (EVs) produced by bacteria, archaea and eukaryotic cells, are increasingly recognized as important mediators of intercellular communication via transfer of a wide variety of molecular cargoes. To characterize the effect of Lp-EVs on THP-1 cells, in particular the immune response, three independent RNAseq libraries were constructed from from THP-1 cells non-infected, incubated with purified wt-EVs or with purified EV from delta-rsmY Legionella bacteria. The RNA deep was purified after 3h of incubation and sequenced using an Illumina platform.

ORGANISM(S): Legionella pneumophila Homo sapiens

PROVIDER: GSE190376 | GEO | 2021/12/10

REPOSITORIES: GEO

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Translocated Legionella pneumophila small RNAs mimic eukaryotic miRNAs to dampen the host immune response

Project description:Extracellular vesicles (EVs) produced by bacteria, archaea and eukaryotic cells, are increasingly recognized as important mediators of intercellular communication via transfer of a wide variety of molecular cargoes. To characterize and identify the RNA molecules enriched in Legionella pneumophila extracellular vesicles (Lp-EVs) we performed RNAseq analyses. Four independent RNAseq libraries were constructed from RNA isolated from purified Lp-EVs and as control from the bacterial pellets, from which the Lp-EVs had been shed and deep sequenced using an Illumina platform. The sequences obtained from the Lp-EVs RNAseq libraries were compared to those obtained from the RNA extracted from the bacterial pellets (Figure 1g). The Lp-EV-sRNA cargo was defined as RNAs for which after normalization at least 1000 reads were sequenced and that showed an enrichment of a log2FC > 5 as compared to the RNAs from the bacterial pellets were. Using these parameters the analysis identified 47 different sRNAs enriched in the Lp-EVs. The 20 highest enriched sRNA that were present in all four biological replicates comprised 8 tRNAs, 7 sRNAs and 5 fragments of mRNA located in the CDS or UTR.

2021-12-06 | GSE159109 | GEO