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Microglia homeostasis mediated by epigenetic ARID1A to regulate neural progenitor cells response in brain development [RNA-seq]

ABSTRACT: Purpose: Purpose:To gain a deeper insight into how microglia homeostasis regulates regulates neurogenesis through epigenetic ARID1A, RNA-sequencing (RNA-seq) was performed to analyze the genome-wide changes by ARID1A deletion in microglia at E14. Methods: The microglia was extracted from E14 of Arid1acKO and Arid1afl/fl mice by fluorescence activated cell sorting. Total RNA was immediately extracted by using the RNAeasy Mini kit (QIAGEN) following the manufacturer’s instructions. Then total RNA was quality controlled and quantified using an Agilent 2100 Bioanalyzer. After converting to cDNA and building library, high-throughput sequencing was performed using the Illumina HiSeq 2500 platform in Annoroad Genomics. Results: Approximately approximately two thousands transcripts showed differential expression between the Arid1afl/fl and Arid1acKO brain cortex, with a fold change ≥2.0 and p value <0.05. Gene ontology (GO) analysis showed that the down-regulated genes were enriched in the terms related to neurogenesis, regulation of cell communication and chemokine-mediated signaling pathway. Up-regulated genes showed a significant enrichment of terms involved in negative regulation of forebrain neuron fate commitment and cell surface receptor signaling pathway involved in cell-cell signaling. These results reflected the importance of ARID1A regulates neurogenesis through remodeling microglia states. Conclusions: We conclude that RNA-seq based transcriptome characterization would provide a framework for understanding how ARID1A regulates neurogenesis through remodeling microglia states brain cortical development.

ORGANISM(S): Mus musculus

PROVIDER: GSE190926 | GEO | 2021/12/16

REPOSITORIES: GEO

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