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The Mediator Kinase Complex Cooperates with Swi/Snf to Regulate Lineage-Resolved Enhancers and Differentiation

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Mus musculus

PROVIDER: GSE191124 | GEO | 2022/10/19

REPOSITORIES: GEO

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The Mediator Kinase Complex Cooperates with Swi/Snf to Regulate Lineage-Resolved Enhancers and Differentiation [RNA-Seq]

Project description:To identify novel functions of Mediator kinases CDK8 and CDK19 in the intestinal epithelium, we generated novel genetic mouse model enabling the individual and compound deletion of CDK8 and CDK19. RNA-sequencing was performed on primary intestinal epithelial cells and murine small intestinal organoids at different time points after CDK8/19 deletion.

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The Mediator Kinase Complex Cooperates with Swi/Snf to Regulate Lineage-Resolved Enhancers and Differentiation [ChIP-Seq]

Project description:Depletion of CDK8 and its paralogue CDK19 in intestinal organoids resulted in the downregulation of genes associated with intestinal secretory cells. To determine the consequence of loss of CDK8/19 in re-defining enhancers we performed ChIP sequencing for MED1, MED12, H3K27Ac and ARID1A. We identified that loss of MED1 and MED12 at super enhancers regulating key intestinal transcription factors and loss of ARID1A binding across intestinal (super-)enhancers.

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