Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression in murine leukemic B cells treated with leukemic extracellular vesicles

ABSTRACT: mRNA profiles of murine leukemic B cells (Eu-TCL1 mice) treated with extracellular vesicles were generated by 3'-sequencing, in triplicate.

ORGANISM(S): Mus musculus

PROVIDER: GSE191186 | GEO | 2022/08/31

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Gene expression in murine chronic lymphocytic leukemia B cells (Eu-TCL1 and Eu-TCL1-Rab27DKO)

Project description:mRNA profiles of leukemic B cells from 8-week-old leukemic (Eu-TCL1 and Eu-TCL1-Rab27DKO) mice were generated by 3'-sequencing, in triplicate.

2022-08-31 | GSE190790 | GEO

Gene expression analysis in murine lymphocyte subsets incubated with control or leukemia-derived extracellular vesicles

Project description:mRNA profiles of Treg, CD4+, CD8+, CD19+ splenic cells from 8-week-old Foxp3 YFP/cre mice incubated with control (WT) or leukemic (TCL1) extracellular vesicles (EV) were analyzed in triplicate.

2022-08-31 | GSE188898 | GEO

Gene expression in murine chronic lymphocytic leukemia

Project description:mRNA profiles of B cells from 8-week-old wild-type (WT) and leukemic (Eu-TCL1) mice were generated by 3'-sequencing, in triplicate.

2021-09-07 | GSE175564 | GEO

Gene expression analysis in lymphocyte subsets from mice injected with control- or leukemia-derived extracellular vesicles

Project description:mRNA profiles of Treg, CD4+, CD8+, CD19+ splenic cells from 8-week-old Foxp3 YFP/cre mice injected with control (WT) or leukemic (TCL1) extracellular vesicles (EV) were analyzed in triplicate.

2022-08-31 | GSE189391 | GEO

Gene expression in murine leukemic B cells treated with leukemic extracellular vesicles

Project description:Gene expression in murine leukemic B cells treated with leukemic extracellular vesicles

| PRJNA789946 | ENA

Gene expression in murine CD8+ T cells treated with leukemia extracellular vesicles

Project description:mRNA profiles of CD8+ T cells from 8-week-old wild-type (Foxp3 YFP/cre) mice treated with control or leukemic sEV were generated by 3'-sequencing, in triplicate.

2022-08-31 | GSE190789 | GEO

Transcriptomic analysis of human regulatory T cells treated with chronic myeloid leukemia-derived extracellular vesicles

Project description:The aim of our study was to evaluate if and how leukemic extracellular vesicles (EVs) modulate human regulatory T cells (Treg). Treg in myeloid leukemias have a pro-leukemic function, but mechanisms driving these cells are unknown. We hypothesised that leukemia-derived extracellular vesicles (EVs) may be a factor that polarizes Treg into pro-leukemic cells. Using bulk RNA sequencing we study transcriptomic differences in human regulatory T cells (3 biological replicates = Treg from 3 different donors) treated with leukemic EVs released by chronic myeloid leukemia K562 cells. We observed significant transcriptomic differences, e.g. in genes responsible for RNA processing and alternative splicing, as well as genes engaged in T cell maturation, origin and activity. Using obtained datasets we also identified transcription factors engaged in this regulation. RNA sequencing data is part of a bigger study, where we study leukemic EVs-Treg interaction using human regulatory T cells, analysis by multicolor flow cytometry, functional studies, in vivo models.

2022-03-30 | GSE180883 | GEO

CD47-dependent immunomodulatory and angiogenic activities of extracellular vesicles produced by T cells

Project description:Intercellular communication is critical for integrating complex signals in multicellular eukaryotes. Vascular endothelial cells and T lymphocytes closely interact during the recirculation and trans-endothelial migration of T cells. In addition to direct cell-cell contact, we show that T cell derived extracellular vesicles can interact with endothelial cells and modulate their cellular functions. Thrombospondin-1 and its receptor CD47 are expressed on exosomes/ectosomes derived from T cells, and these extracellular vesicles are internalized and modulate signaling in both T cells and endothelial cells. Extracellular vesicles released from cells expressing or lacking CD47 differentially regulate activation of T cells induced by engaging the T cell receptor. Similarly, T cell-derived extracellular vesicles modulate endothelial cell responses to vascular endothelial growth factor and tube formation in a CD47-dependent manner. Uptake of T cell derived extracellular vesicles by recipient endothelial cells globally alters gene expression in a CD47-dependent manner. CD47 also regulates the mRNA content of extracellular vesicles in a manner consistent with some of the resulting alterations in target endothelial cell gene expression. Therefore, the thrombospondin-1 receptor CD47 directly or indirectly regulates intercellular communication mediated by the transfer of extracellular vesicles between vascular cells. HuVEC cells were cocultured with exosomes derived either from Jurkat or JinB8 cells culture media. Each condition was done in triplicate. Also, Exosome RNA from Jurkat or JINB8 cells were compared to each other in triplicate.

2014-09-01 | E-GEOD-56825 | biostudies-arrayexpress

Impact of Group Psychological Interventions on Extracellular Vesicles in People Who Had Cancer

Project description:Background: There is some evidence demonstrating the effect of psychological interventions in improvements in health biological parameters. To best of our knowledge, no study had addressed the impact of any psychological intervention on extracellular vesicles. In addition, Mindfulness-Based Cognitive Therapy (MBCT) and Emotion Focused Therapy for Cancer Recovery (EFT-CR) in the group have never been explored regarding extracellular vesicles and the effectiveness of these was not compared yet. Objectives: 1. To explore and compare the effect of MBCT and EFT-CR on biological parameters and psychological variables in distressed people who have had breast, prostate and colorectal cancer; 2. In addition, we will explore the acceptability through recruitment and retention rates of MBCT and EFT-CR in group and evaluate whether these interventions are appropriate for a larger clinical trial. Methods: The design of this study is a parallel randomized controlled trial. Participants will be randomized into MBCT, EFT-CR or usual care. Outcome measures will be assessed before, at the end of the intervention (8 weeks) and follow-ups (24 and 52 weeks from the baseline moment). Hypotheses: The researchers expected that both interventions will have an effect on extracellular vesicles and other study biomarkers as well as improvements in psychological outcomes, compared to treatment as usual (TAU) group. Regarding the comparative effectiveness, we did not have evidence to hypothesize which one of the interventions will be superior in both biological (extracellular vesicles) and psychological outcomes. Contribution for practice: The results of this preliminary study would permit to know if there are benefits of these psychological interventions on changes in extracellular vesicles and on psychological outcomes related to health. In addition, this study will permit to determine the acceptability of conducting a larger randomized controlled trial.

| 2332322 | ecrin-mdr-crc

sRNAs from Neisseria extracellular vesicles

Project description:Similar to bacterial proteins that are targeted to distinct macrophages organelles via extracellular vesicles, we propose that these vesicles also traffic small RNAs to modulate specific host factors. To test this, we aim to sequence extracellular vesicle derived sRNA, and whole bacterial small RNAs to determine selectivity, and to identify their bacterial and mammalian targets (Experimental Plan in Table-1). For this we will collect highly purified vesicles from N. gonorrhoeae (strain MS11A). We will also treat mouse derived primary macrophages with extracellular vesicles and compare their RNA response to untreated macrophages (Table-2). This will provide novel insights into how macrophages respond to N. gonorrhoeae infections. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/

2016-03-01 | E-ERAD-439 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data