HSP70 binds RNA and regulates transcription by RNA Polymerase III [FLASH-seq]

ABSTRACT: We examined the RNA binding ability of the 70-kDa heat shock protein (HSP70) family member HSPA1A and HSP8 and identified the RNA species bound to HSP70 or HSP8 in vivo. We used a UV-crosslinkiung and immunoprecipitation based approach named FLASH-seq (Akta? et al., 2017) using 3xFLAG-Histidine-Biotin tagged ectopically expressed protein in HEK293T cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE191242 | GEO | 2024/01/17

REPOSITORIES: GEO