Genomics

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Effect of cyclic tensile stretch on meniscus cells in the presence and absence of IL-1


ABSTRACT: Meniscus injuries are highly prevalent and are linked to the development of post-traumatic osteoarthritis (PTOA). The inflammatory cytokine IL-1 is elevated in synovial fluid following knee injuries, causes degradation of meniscus tissue, and inhibits meniscus repair. Cyclic tensile stretch has been shown to modulate the expression of pro-inflammatory cytokines and degradative enzymes induced by IL-1; however, there remains a dearth of knowledge on global effects of loading on meniscus cell phenotype and transcriptomic profiles. In this study, we performed mRNA-Seq on meniscus cells isolated from inner and outer zone regions of porcine menisci subjected to cyclic tensile strain in the presence and absence of IL-1 to identify cellular responses to mechanical load, identify differences in response to load based on zonal characteristics, and identify IL-1 induced inflammatory responses modulated by load. Overall design: RNA-Seq of inner and outer zone porcine meniscus cells explants subjected to 5% cyclic tensile strain at 0.5Hz in the presence or absence of IL-1 (n=3/group)

INSTRUMENT(S): Illumina NovaSeq 6000 (Sus scrofa)

ORGANISM(S): Sus scrofa  

SUBMITTER: Amy McNulty  

PROVIDER: GSE191321 | GEO | 2021-12-23

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
GSE191321_2021-10-08_Cell_Stretch_RawCounts.xlsx Xlsx
GSE191321_2021-12-20_Cell_Stretch_Normalized_Counts.xlsx Xlsx
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