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NPA-induced vascular overgrowth in Arabidopsis

ABSTRACT: We used N-(1-naphthyl) phthalamic acid (NPA)-induced vascular overgrowth in Arabidopsis leaves to look for differential up-regulation of genes in NPA-treated tissues that may be involved in vascular differentiation. Arabidopsis thaliana Col-0 plants were grown for approximately 2 weeks on solid ATS medium (1) containing a final concentration of 10 um NPA (dissolved in DMSO) or an equivalent volume of DMSO (control). At this stage plants had approximately 6 rosette leaves. RNA was prepared from entire shoot tissues of control (DMSO) or NPA-treated plants.(1) Lincoln et al., 1990. Plant Cell 2: 1071-1080.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE19262 | GEO | 2009/12/05

SECONDARY ACCESSION(S): PRJNA120929

REPOSITORIES: GEO

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NPA-induced vascular overgrowth in Arabidopsis

Project description:We used N-(1-naphthyl) phthalamic acid (NPA)-induced vascular overgrowth in Arabidopsis leaves to look for differential up-regulation of genes in NPA-treated tissues that may be involved in vascular differentiation. Arabidopsis thaliana Col-0 plants were grown for approximately 2 weeks on solid ATS medium (1) containing a final concentration of 10 um NPA (dissolved in DMSO) or an equivalent volume of DMSO (control). At this stage plants had approximately 6 rosette leaves. RNA was prepared from entire shoot tissues of control (DMSO) or NPA-treated plants.(1) Lincoln et al., 1990. Plant Cell 2: 1071-1080. 2 samples were used in this experiment.

2009-12-05 | E-GEOD-19262 | biostudies-arrayexpress

Rice root: Control vs auxin transport inhibitor N-1-naphthylphthalamic (NPA) treatment; WT vs iaa13 mutant.

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Snell Dwarf Response to 3-NPA

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Aqueous and DMSO extracts from Annona muricata leaves protect from ferroptosis to both HT-1080 fibrosarcoma cells and HMEC-1 endothelial cells.

Project description:Graviola (Annona muricata) is a tropical plant with many traditional ethnobotanic uses and pharmacologic applications. A metabolomic study of both aqueous and DMSO extracts from Annona muricata leaves recently allowed us to identify dozens of bioactive compounds. In the present study, we use a proteomic study to reveal new bioactivities of these leave extracts on both conditioned media and extracts of HT-1080 fibrosarcoma treated cells. Our results reveal the complete sets of deregulated proteins after treatment with aqueous and DMSO extracts from An-nona muricata leaves. Functional enrichment analysis of proteomic data suggests deregulation of cell cycle and iron metabolism, which are experimentally validated. Additional experimental data reveal that these extracts protect from ferroptosis to both HT-1080 fibrosarcoma cells and HMEC-1 endothelial cells.

2023-10-24 | PXD042354 | Pride

Transcriptome analysis of tomato in response to treatment with neutralized phosphorous acid (NPA)

Project description:Phosphonate related fungicides such as neutralized phosphorous acid (NPA) are effective for the control of plant diseases, especially those caused by Phytophthora and other oomycetes. It has been suggested that phosphonate may induce plant resistance, however, the mechanism is not clear thus far. We performed microarray analysis using Affymetrix Tomato Genome Array to identify tomato genes that are differentially expressed in response to NPA treatment.

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Expression data from NPA treated stems of Arabidopsis thaliana.

2011-09-01 | GSE24763 | GEO

Expression data from 1-N-naphthylphthalamic acid (NPA) treated stems of Arabidopsis thaliana

Project description:In-vivo induced establishment and activity of the interfascicular cambium in Arabidopsis thaliana stems under NPA treatments. We used microarrays to detail the global programme of gene expression underlying the establishment and activity of the interfascicular cambium. 3 mm stem pieces were collected after being in treated with NPA for a week in order to stop the polar auxin transport and induce secondary growth. Collected samples were used for RNA extraction and hybridization on Affymetrix microarrays. We aimed to obtain genes responsible for auxin-induced cambium establishment and activity.

2011-08-31 | E-GEOD-24763 | biostudies-arrayexpress

Arabidopsis thaliana

Project description:NPA-induced vascular overgrowth in Arabidopsis

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Expression data of Medicago truncatula skl1-1 roots treated with S. meliloti wild-type or auxin transport inhibitors

Project description:Rhizobium and allied bacteria form symbiotic nitrogen-fixing nodules on legume roots. Plant hormones appear to play a role in nodule formation. We treated Medicago truncatula roots with auxin transport inhibitors (ATIs) N-(1-naphthyl)phthalamic acid (NPA) and 2,3,5-triiodobenzoic acid (TIBA) to induce the formation of pseudonodules. We compared the transcriptional responses of M. truncatula roots treated with ATIs to roots inoculated with Sinorhizobium meliloti. The transcriptional response of M. truncatula roots 1 and 7 days after ATI treatment were opposite to roots treated with S. meliloti.

2011-03-26 | GSE28172 | GEO

Expression data of Medicago truncatula Jemalong A17 roots treated with S. meliloti exoA mutant or auxin transport inhibitors

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