RNAseq of A431 cells with Fungi or Calphostin C treatment

ABSTRACT: To determine the profile of the gene expression changes of human SCC cell line A431 under the treatment of cladosporides and its metabolite- calphostinC, we conducted the next generation sequencing on mRNA from the cell line after 5h treatment of the fungi and chemical. Extracted RNA samples were pooled and sequenced on NextSeq using Illumina TruSeq stranded mRNA Library Prep and paired-end sequencing. After analyzing, we performed GSEA analysis and found many DEGs are enriched in JAK/STAT3 pathway and glucose-related metabolic pathways. Heatmaps are also created based on these pathways. To confirm the signicant alterations under treatments, we also performed real time PCR for several related genes and got the consistent results with the sequencing.

ORGANISM(S): Homo sapiens

PROVIDER: GSE192654 | GEO | 2026/08/20

REPOSITORIES: GEO