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Microarray Analysis of Retinal Endothelial Tip Cells Identifies CXCR4 as a Mediator of Tip Cell Morphology and Branching

ABSTRACT: The development of the vertebrate vascular system is mediated by both genetic patterning of vessels and by angiogenic sprouting in response to hypoxia. Both of these processes depend on the detection of environmental guidance cues by endothelial cells. A specialized subtype of endothelial cell known as the tip cell is believed to be involved in the detection and response to these cues, but the molecular signaling pathways utilized by tip cells to mediate tissue vascularization remain largely uncharacterized. To identify genes critical to tip cell function, we have developed a method to isolate them using laser capture microdissection, permitting comparison of RNA extracted from endothelial tip cells to that of endothelial stalk cells using microarray analysis. Genes enriched in tip cells include ESM-1, Angiopoietin-2, and SLP-76. CXCR4, a receptor for the chemokine SDF-1, was also identified as a tip cell-enriched gene, and we provide evidence for a novel role for this receptor in mediating tip cell morphology and vascular patterning in the neonatal retina.

ORGANISM(S): Mus musculus

PROVIDER: GSE19284 | GEO | 2010/03/17

SECONDARY ACCESSION(S): PRJNA120967

REPOSITORIES: GEO

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Specialized endothelial tip cells guide neuroretina vascularization and blood-retina-barrier formation

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Endothelin2 signaling in the neural retina promotes the endothelial tip cell state and inhibits angiogenesis

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2014-01-01 | GSE50059 | GEO

CD34 marks angiogenic tip cells in human vascular endothelial cell cultures.

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