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Substantial equivalence in transgenic barley

ABSTRACT: In this study, possible adverse effects of transgene expression in field-grown barley were assessed in relation to the influence of genetic background and to the impact of interaction with arbuscular mycorrhiza fungi. The deposited microarray data originate from a parallel transcript profiling, metabolome profiling and metabolic fingerprinting approach with the wild type cultivars Golden Promise (GP) and Baronesse (B) as well as barley transgenics with i) seed-specific expression of (1,3-1,4)-ß-glucanase (GluB) being introgressed from the Golden Promise (GP) into the cultivar Baronesse (B) and ii) ubiquitous expression of codon-optimized Trichoderma harzianum endochitinase (ChGP). Our conclusion from the results is that cultivar-specific differences and even the presence of few introgressed alleles exceed the effects on transcriptome and metabolome caused by transgene expression. Mycorrhization was shown to have more prominent effects on the metabolome than on the transcriptome.

ORGANISM(S): Hordeum vulgare

PROVIDER: GSE19296 | GEO | 2010/03/03

SECONDARY ACCESSION(S): PRJNA120879

REPOSITORIES: GEO

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Substantial equivalence in transgenic barley

Project description:In this study, possible adverse effects of transgene expression in field-grown barley were assessed in relation to the influence of genetic background and to the impact of interaction with arbuscular mycorrhiza fungi. The deposited microarray data originate from a parallel transcript profiling, metabolome profiling and metabolic fingerprinting approach with the wild type cultivars Golden Promise (GP) and Baronesse (B) as well as barley transgenics with i) seed-specific expression of (1,3-1,4)-ß-glucanase (GluB) being introgressed from the Golden Promise (GP) into the cultivar Baronesse (B) and ii) ubiquitous expression of codon-optimized Trichoderma harzianum endochitinase (ChGP). Our conclusion from the results is that cultivar-specific differences and even the presence of few introgressed alleles exceed the effects on transcriptome and metabolome caused by transgene expression. Mycorrhization was shown to have more prominent effects on the metabolome than on the transcriptome. Hordeum vulgare plants were cultivated in the field in 2007 at Giessen Experimental station (165m elevation over NN, 1540h sunshine and 650mm precipitation per year in average) in replicated blocks, each consisted of eight 0.8 m × 0.5 m plots in which genotypes were randomly distributed and half of the plots were pretreated with Amykor® (containing the arbuscular fungi Glomus intraradices and Glomus mosseae). Ten pools of leaf material of at least 10 leaves per pool were sampled in the middle of the light period four months after planting. Two replicates of each genotype were subjected to microarray analysis.

2010-03-02 | E-GEOD-19296 | biostudies-arrayexpress

Transcription profiling of barley seedling leaves from Golden Promise and Morex cultivars and in F1 hybrids

Project description:We measured mRNA abundance in the seedling leaves of the barley genotypes Golden Promise and Morex and in F1 hybrids generated using either Golden Promise as a maternal genotype and Morex as paternal or other way around. 3 biological replicates each, total 12 chips.

2007-12-31 | E-TABM-110 | biostudies-arrayexpress

GoldenPromiseAssembly

Project description:Barley cv. Golden Promise reference assembly

| PRJEB34691 | ENA

Chloroplast activity provides invitro regeneration capability in contrasting cultivars

Project description:Existence of potent in vitro regeneration system is a prerequisite for efficient genetic transformation and functional genomics of crop plants. Little is known why only some cultivars in crop plants are tissue culture friendly? In this study, contrasting barley cultivars Golden Promise (GP) and DWRB91 (D91) were utilized for digging the molecular basis of regeneration efficiency. Multiomics studies involving transcriptomics, proteomics, metabolomics, and biochemical analysis were performed using GP and D91 callus to unravel the regulatory mechanisms. Transcriptomics analysis revealed 1487 differentially expressed genes (DEGs), in which 795 DEGs were upregulated and 692 DEGs were downregulated in the GP-D91 transcriptome. Genes encoding proteins localized in chloroplast and involved in ROS generation were upregulated in the embryogenic calli of GP. Moreover, proteome analysis by LC-MS revealed 3062 protein groups and 16989 peptide groups, out of these 1586 protein groups were differentially expressed proteins (DEPs). Eventually, GC-MS based metabolomics analysis also revealed the higher activity of plastids and alterations in key metabolic processes such as sugar metabolism, fatty acid biosynthesis, and secondary metabolism. Higher accumulation of sugars, amino acids and metabolites corresponding to lignin biosynthesis were observed in GP as compared to D91.

2022-07-31 | GSE206271 | GEO

Transcription profiling of barley under salinity stress and JA treatment

Project description:Expression Data of Barley Crown and Growing Point Tissue Under Salt Stress abd JA treatment Experiment Overall Design: Barley genotype Golden Promise was used for expression anlaysis using the tissue from crown and growing point under control, salt stressed, JA treatment and JA pretreatment followed by salt stress

2008-06-13 | E-GEOD-5605 | biostudies-arrayexpress

Transcription profiling of seedling leaves from 8 barley genotypes: Morex, Steptoe, Golden Promise, Optic, Haruna Nijo, Barke, OWB-D and OWB-R

Project description:We measured mRNA abundance in the seedling leaves of 8 barley genotypes; Morex, Steptoe, Golden Promise, Optic, Haruna Nijo, Barke, OWB-D and OWB-R. 3 biological replicates each, total 24 chips.

2007-12-31 | E-TABM-113 | biostudies-arrayexpress

Array-based genotyping and expression analysis of barley cv. Maythorpe and Golden Promise

Project description:Salt Stress response of salt-tolerant genotype Golden Promise compared to Maythorpe Barley1 GeneChip was used to find differential expression between two barley genotypes under control and salt stress conditions at vegetative stage of growth Keywords: genotype and treatment comparison

2007-03-31 | GSE6325 | GEO

Cold induced leaf transcriptome profiling of the TaCBF14 and TaCBF15 transgenic barley lines

Project description:Wild type Golden Promise barley and the frost tolerant TaCBF14 and TaCBF15 transgenic barley lines were grown in plant growth chamber in the Phytotron of ELKH ATK Agricultural Institute, Martonvasar, Hungary. After three weeks in control conditions, plants were treated by short-term cold-treatment. Samples were collected under control condition and after the short-term cold-treatment too.

2021-11-10 | E-MTAB-10781 | biostudies-arrayexpress

Barley

Project description:A wild barley accession WB-1 and a barley cultivar Golden Promise Raw sequence reads

| PRJNA485436 | ENA

RNA-seq of drought response and drought recovery in barley (cv. Golden Promise) on a lysimeter platform.

Project description:Determination of changes in expression of genes and pathways as a consequence of drought treatment and following rewatering in barley cv. Golden Promise. RNAs were collected following stomatal closure (theta-critical point) and following rewatering from leaves as well as under fully watered conditions. The plants were grown under greenhouse conditions.

2024-02-01 | E-MTAB-12732 | biostudies-arrayexpress

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