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Hepatocellular carcinomas (HCC) in mice transduced in utero with feline immunodeficiency virus-based vectors (expression)

ABSTRACT: Fetal mice (16 days gestation) were administered feline immunodeficiency virus (FIV)-based lentiviral viral particles containing the gene encoding GFP. Six liver tumors developed in three mice between the ages of 273 and 484 days, each mouse developed 2 tumors. These tumors and non-tumorous liver tissue from the same animals and animals that did not develop tumors and untransduced controls were harvested and microarrays were performed on total RNA extracted from these samples. We were interested in investigating the link between lentiviral integration and gene expression. Keywords: Comparison of HCC with non-tumorous liver tissue adjacent to tumor.

ORGANISM(S): Mus musculus

PROVIDER: GSE19306 | GEO | 2013/09/11

SECONDARY ACCESSION(S): PRJNA120899

REPOSITORIES: GEO

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Hepatocellular carcinomas (HCC) in mice transduced in utero with feline immunodeficiency virus-based vectors (expression)

Project description:Fetal mice (16 days gestation) were administered feline immunodeficiency virus (FIV)-based lentiviral viral particles containing the gene encoding GFP. Six liver tumors developed in three mice between the ages of 273 and 484 days, each mouse developed 2 tumors. These tumors and non-tumorous liver tissue from the same animals and animals that did not develop tumors and untransduced controls were harvested and microarrays were performed on total RNA extracted from these samples. We were interested in investigating the link between lentiviral integration and gene expression. Keywords: Comparison of HCC with non-tumorous liver tissue adjacent to tumor. Fourteen samples were analyzed in total: Two tumors from each of three mice (6 samples); surrounding non-tumorous liver tissue from each of these three mice (3 samples); two female mice and one male mouse that were transduced and did not develop tumors (3 samples); one female and one male untransduced mouse (2 samples). The samples were performed in two runs: one containing the samples from female mice and the second containing samples from male mice.

2013-09-11 | E-GEOD-19306 | biostudies-arrayexpress

Hepatocellular carcinomas (HCC) in mice transduced in utero with feline immunodeficiency virus-based vectors (CGH)

Project description:Fetal mice (16 days gestation) were administered FIV-based lentiviral viral particles containing the gene encoding EGFP. Six liver tumors developed in three mice (1 male, 2 females) between the ages of 273 and 484 days, each mouse developed 2 tumors. These tumors and non-tumorous liver tissue from the same animals were harvested and aCGH was performed on genomic DNA extracted from these samples. We were interested in investigating the link between lentiviral integration and chromosomal aberrations. Two tumors from each mouse (1 male, 2 females) were each compared to non-tumorous tissue surrounding the tumors.

2013-09-11 | E-GEOD-25900 | biostudies-arrayexpress

Hepatocellular carcinomas (HCC) in mice transduced in utero with feline immunodeficiency virus-based vectors (CGH)

2013-09-11 | GSE25900 | GEO

MicroRNA profile analysis of feline kidney cell line infected with Feline Panleukopenia Virus using deep sequencing

Project description:Purpose:MicroRNAs (miRNAs) are members of a rapidly growing class of small endogenous non-coding RNAs that play crucial roles in post-transcriptional regulator of gene expression in many biological processes. Feline Panleukopenia Virus (FPV) is a highly infectious pathogen that causes severe disease in pets, economically important animals and wildlife in worldwide. However, the molecular mechanisms underlying the pathogenicity of FPV have not been completely clear. To study the involvement of miRNAs in the FPV infection process, miRNAs expression profiles were identified via deep sequencing in the feline kidney cell line (F81) infected and uninfected with FPV. Methods:miRNA-sequencing analysis was performed on an Illumina Hiseq 2500 (LC Sciences, USA) following the vendor's recommended protocol Results:As a result, 673 known miRNAs belonging to 210 families and 278 novel miRNAs were identified. Then we found 57 significantly differential expression miRNAs by comparing the results between uninfected and FPV-infected groups. Furthermore, stem-loop qRT-PCR was applied to validate and profile the expression of the randomly selected miRNAs; the results were consistent with those by deep sequencing. Furthermore, the potential target genes were predicted. The target genes of differential expression miRNAs were analyzed by GO and KEGG pathway. Conclusions:The identification of miRNAs in feline kidney cell line before and after infection with Feline Panleukopenia Virus will provide new information and enhance our understanding of the functions of miRNAs in regulating biological processes.

2020-12-20 | GSE122381 | GEO

Expression data from feline liver tissue

Project description:This study looks at the effect of dietary manipulation on the development of hepatic steatosis and changes in hepatic gene expression in a feline model. We used microarray analysis to examine changes in hepatic gene transcription in response to Trans fat, High Fructose Corn Syrup (HFCS) and/or Monosodium Glutamate (MSG) in the domestic cat. The use of human Affymetrix arrays for the study of feline gene expression has previously been validated by Dowling and Bienzle, 2005, Journal of General Virology. 86(Pt 8), 2239-48 (PMID 16033971). Our study animals were bred from female Felis catus previously placed on one of 4 different dietary regimens for a period of 3 weeks prior to mating. The four dietary regimens used in this study were: [1] Standard Chow Control feline diet (Test Diet Purina catalog #5003); [2] MSG diet consisting of Control diet with 1.125% added Monosodium Glutamate (Diet A: Test Diet Purina catalog #5C1J); [3] Trans-fat/HFCS diet, containing 8.6% Trans fat and 24% HFCS (Diet B: Test Diet Purina catalog #5B4K); and [4] Trans-fat/HFCS and MSG diet, containing 8.6% Trans fat, 24% HFCS and 1.125% MSG (Diet C: Test Diet Purina catalog #5C1H). Following mating, the 4 groups of dams were maintained on their respective diets throughout the gestation and nursing period. Male offspring used in the following experiments were weaned onto the same diets and maintained on their respective dietary regimens until they reached 9 months of age. Hepatic tissues (4-5 per diet group) were used for RNA extraction and hybridization on Affymetrix microarrays.

2014-06-11 | E-GEOD-30040 | biostudies-arrayexpress

Feline Metagenome

Project description:Feline Metagenome

| PRJNA616033 | ENA

Transcriptomic analysis of feline chronic gingivostomatitis (FCGS)

Project description:The purpose of this study was to characterize the transcriptomic alterations accompanying the inflammation involved in feline chronic gingivostomatitis (FCGS). Towards this goal next-generation sequencing (NGS)-based gene expression profiling (RNA-Sequencing; RNA-Seq) was performed on matched pairs of FCGS diseased and healthy tissues obtained from three feline subjects.

2020-09-25 | GSE149881 | GEO

Expression data from feline liver tissue

2014-06-11 | GSE30040 | GEO

feline metagenome

Project description:microbiota in feline kidney disease

| PRJNA413077 | ENA

Human and feline adipose-derived mesenchymal stem cells have comparable immunomodulatory functions

Project description:Adipose-derived mesenchymal stem cells (ASCs) are a promising cell therapy to treat inflammatory and immune-mediated diseases. Development of appropriate pre-clinical animal models is critical to determine safety and attain early efficacy data for the most promising therapeutic candidates. Naturally occurring diseases in cats already serve as valuable models to inform human clinical trials in oncologic, cardiovascular and genetic diseases. The objective of this study was to complete a comprehensive side-by-side comparison of human and feline ASCs with an emphasis on their immunomodulatory capacity and transcriptome. Similar to human ASCs, feline ASCs were highly proliferative at low passages and fit the minimal criteria of multipotent stem cells including a compatible surface protein phenotype, osteogenic capacity and normal karyotype. Like ASCs from all species, feline ASCs inhibited mitogen activated lymphocyte proliferation in vitro, with or without direct ASC-lymphocyte contact. Feline ASCs mimic human ASCs in their mediator secretion pattern including prostaglandin E2, indoleamine 2,3 dioxygenase, transforming growth factor beta and interleukin-6, all augmented by interferon gamma secretion by lymphocytes. The transcriptome of 3 unactivated feline ASC lines were highly similar. Functional analysis of the most highly expressed genes highlighted processes including: 1) the regulation of apoptosis, 2) cell adhesion, 3) response to oxidative stress, and 4) regulation of cell differentiation. Finally, feline ASCs had a similar gene expression profile to noninduced human ASCs. These data will help inform clinical trials using cats with naturally occurring diseases as surrogate models for human clinical trials in the regenerative medicine arena.

2017-03-12 | GSE94773 | GEO

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