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Single-cell RNA sequencing characterizes the comparision between Granulocyte-colony stimulating factor (G-CSF) primed human bone marrow and unstimulated bone marrow

ABSTRACT: Purpose: The goal of the study is to obatin a comprehensive landscape of G-CSF primed bone marrow (G-BM) and to deciphering the underlying mechanisms of immune modulatory effect induced by G-CSF treatment. Methods: We recruited two healthy donors and treated them with recombinant G-CSF at a dosage of 5μg/kg body weight per day for 5 consecutive days. The bone marrow samples were collected by aspiration on the fourth day of G-CSF treatment. Bone marrow samples from healthy donors were treated with lysing solution to remove erythrocytes and were then labeled with antibodies. Through flow sorting, the final cells used for single-cell RNA sequencing (scRNA-seq) contained CD45+CD66b- population (90%), CD66b+ population (5%), and CD45- population (5%). Results and Conclusion: Through unbiased analysis, our data systematically showed the alterations in the transcriptional landscape of hematopoietic cells in G-BM, and illustrated the mechanisms of hyporesponsiveness of T cells and natural killer (NK) cells caused by G-CSF stimulation.

ORGANISM(S): Homo sapiens

PROVIDER: GSE193138 | GEO | 2022/06/23

REPOSITORIES: GEO

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RNA-seq of CD11bhiLy6ChiLy6Glo bone marrow cells and CD11bmidLy6CmidLy6Glo bone marrow cells against CD11bloLy6CloLy6Glo bone marrow cells

Project description:CD11bloLy6CloLy6Glo cells were sorted from the steady-state bone marrow (BM) of B6 mice, i.e. cells cultured for 5 d without human BM-derived mesenchymal stromal cells (MSCs) in the absence of GM-CSF. CD11bhiLy6ChiLy6Glo cells were isolated from BM cells cultured for 5 d under GM-CSF incubation (40 ng/ml) but without MSC coculture. CD11bmidLy6CmidLy6Glo cells were sorted from GM-CSF-stimulated, MSC-cocultured BM cells.

2020-05-01 | E-MTAB-8975 | biostudies-arrayexpress

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Expression data of colony-stimulating factor (CSF) grown bone marrow derived cells

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Global miRNA Expression of Bone Marrow Mesenchymal Stem/Stromal Cells in Fanconi Anemia Patients Before Transplantation and Unpaired Bone Marrow Donors

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2022-09-01 | GSE183226 | GEO

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