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Cell type specific transcriptome profiling reveals a role for thioredoxin in neutrophils and tumor initiation

ABSTRACT: We report the application of Translating Ribosomal Affinity Purification (TRAP) seq for identifying cell type specific gene expression in the zebrafish larvae. Using cell specific promoters to drive an EGFP-tagged ribosomal subunit, we were able to identify translating mRNA transcripts in keratinocytes (krt4), neutrophils (lyz) and macrophages (mpeg) during early cancer transformation. In our zebrafish model of HRasG12V -transformed keratinocytes, we identified 56 differentially expressed genes in keratinocytes, 53 genes in neutrophils, and no differentially expressed genes found in macrophages, compared to control. These data indicate activation of gene expression pathways in distinct cell types following early keratinocyte transformation and validate the use of TRAPseq in the zebrafish model system.

ORGANISM(S): Danio rerio

PROVIDER: GSE193591 | GEO | 2022/02/04

REPOSITORIES: GEO

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Generation of a binary transgenic zebrafish model to study myeloid gene regulation in response to pre-neoplastic melanocytes

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2018-05-30 | GSE96534 | GEO

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2023-10-11 | PXD039560 | JPOST Repository

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2015-07-23 | E-GEOD-66054 | biostudies-arrayexpress

MicroRNA-203 represses selection and expansion of oncogenic HRas transformed tumor initiating cells [array]

2015-07-23 | E-GEOD-66055 | biostudies-arrayexpress

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