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Collagen-GAG Biocompatibility Tests

ABSTRACT: All mRNA was isolated after 8 hours of culture time in each of three culture conditions. (1) TCPS Plate, (2) Collagen-GAG 2 dimensional coated plate and (3) collagen-GAG three dimensional mesh. Keywords: ordered

ORGANISM(S): Homo sapiens

PROVIDER: GSE1937 | GEO | 2004/11/09

SECONDARY ACCESSION(S): PRJNA90427

REPOSITORIES: GEO

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Transcription profiling of human fibroblasts grown on TCPS plates, Collagen-GAG 2 D plate and Collagen-GAG 3D mesh

Project description:All mRNA was isolated after 8 hours of culture time in each of three culture conditions. (1) TCPS Plate, (2) Collagen-GAG 2 dimensional coated plate and (3) collagen-GAG three dimensional mesh.

2008-06-11 | E-GEOD-1937 | biostudies-arrayexpress

Transcription profiling of human fibroblasts grown in petri dishes vs. attached to a tissue engineering scaffold sampled at 7 timepoints.

Project description:A goal of tissue engineering is to produce a scaffold material that will guide cells to differentiate and regenerate functional replacement tissue at the site of injury. Little is known about how cells respond on a molecular level to tissue engineering scaffold materials. In this work we used oligonucleotide microarrays to interrogate gene expression profiles associated with cell-biomaterial interactions. We seeded collagen-glycosaminoglycan meshes, a widely used tissue engineering scaffold material, with human IMR-90 fibroblasts and compared transcript levels with control cells grown on tissue culture polystyrene. Genes involved in cell signaling, extracellular matrix remodeling, inflammation, angiogenesis and hypoxia were all activated in cells on the collagen-GAG mesh. Understanding the impact of a scaffold on attached cells will facilitate the design of improved tissue engineering materials.

2007-10-26 | E-GEOD-6432 | biostudies-arrayexpress

Collagen 1 Hydrogel Dynamic Loading Tests

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Functional Changes of HepG2 cells by VECELL 3-D Culture

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2015-09-30 | GSE41270 | GEO

Homo sapiens

Project description:Collagen-GAG Biocompatibility Tests

| PRJNA90427 | ENA

Project description:IMR-90 cells: Tissue Culture Polystyrene or Collagen Mesh Time Course

| PRJNA99513 | ENA

IMR-90 cells: Tissue Culture Polystyrene Time Course

Project description:IMR-90 Human Fibroblasts were seeded onto TCPS dishes. mRNA was isolated at 1, 2, 4, 8, 12, 24, 48 and 96 hours. This time course was used as a control for gene expression studies on collagen/chondroitin sulfate tissue engineering scaffold materials using the same cell line. Keywords = tissue culture plates Keywords: time-course

2003-09-10 | GSE410 | GEO

RNA Sequencing of Novel HIV RNA TAR-gag and Host Genome of EVs from HIV-1 Infected Cells

Project description:We performed a 3' RACE of a novel HIV RNA TAR-gag in order to determine the sequence of the RNA at the 3' end. Our data had shown that TAR-gag was potentially a noncoding RNA and our hypothesis was that TAR-gag ended somewhere prior to the end of the gag region of the HIV genome. The 3' RACE experiment showed that TAR-gag actually consists of four different RNA clusters, the longest of which ends at 615 bases from the transcription start site; this is in the middle of the p17 region of the gag gene. In addition, we sequenced all host RNAs in the EVs.

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Expression of enzymes involved in gag synthesis and degradation in cartilage aging and arthritis

Project description:Glycoarrays are used to identify differences in the expression of enzymes involved in gag synthesis and degradation in cartilage aging and arthritis. This experiment includes 3 samples from donors with and without growth factor stimulation. Samples are from human biopsy/necropsy origin. Human knee chondrocytes were grown in monolayer culture from 3 donors unstimulated or stimulated with TGFß1 (10ng/ml) for 6 hours.

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Determination of Gag stoichiometry for different production platforms by PRM analysis

Project description:In this work we established an analytical method of characterization for the Gag protein core and clarify the current variability of Gag stoichiometry in HIV-1 VLPs depending on the cell-based production platform, directly determining the number of Gag molecules per VLP in each case. Three Gag peptides have been validated to quantify the number of monomers using parallel reaction monitoring (PRM)

2022-12-07 | PXD021278 | Pride

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