Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Quantification of transcriptome levels in mouse spermatogonia TET1 overexpression group and control cells by RNA-seq

ABSTRACT: By analyzing the sequencing results of RNA-seq, we found that TET1 maintains self-renewal of mouse spermatogonia and promotes cellular transcription and enhances cellular activity, and we identified key differential genes that are specifically affected by TET1 and the mechanism of interaction between these key differential genes, providing a scientific basis for studying spermatogenesis, revealing the causes of spermatogenic disorders, and elucidated the mechanisms, which may contribute to the understanding of male reproductive disorders.

ORGANISM(S): Mus musculus

PROVIDER: GSE193717 | GEO | 2022/01/17

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

TET1-JMJD3-H3K27me3 targets and regulates Pramel3 to affect spermatogonia self-renewal and proliferation and differentiation

Project description:Histone modification and DNA methylation among epigenetic modifications play a coordinated role in the regulation of spermatogonia. Tet methylcytosine dioxygenase 1 (TET1) can either act as a DNA demethylase, converting 5-methylcytosine to 5-hydroxymethylcytosine or regulate gene expression by forming a complex with other proteins. Increased levels of H3K27me3 will result in transcriptional repression and reduced or silenced protein expression. However, the mechanism by which TET1 mediates the regulation of H3K27me3 in spermatogonia and the target genes that act is not clear. The results of this study showed that the expression of self-renewal-related gene GFRA1 and proliferation-related gene PCNA were up-regulated after JMJD3 overexpression. Moreover, it was demonstrated that TET1 and JMJD3 can interact with H3K27me3 by forming a protein complex, and the CHIP-Seq sequencing results analyzed that TET1 targets and regulates the Pramel3 gene when interacting with H3K27me3, and TET1 overexpression promotes the up-regulation of Pramel3 expression. Our study identified a novel link between TET1 and H3K27me3 and established a TET1-JMJD3-H3K27me3-Pramel3 regulatory axis to regulate spermatogonia self-renewal, proliferation and differentiation. It provides a new idea to further investigate the mechanism of spermatogenesis and spermatogenic disorders from the epigenetic level.

2023-05-24 | GSE233150 | GEO

RNA-seq data of Spermatogonia cells of Tet1-ko and WT

Project description:We analyzed 3 repeats of 12 samples of mouse spermatogonia cells including 3-month-old WT and 3 month-old Tet1, 11-month-old WT and 11-month-old Tet1, aimed to find Tet1 function on male fertility.

2020-01-18 | GSE140014 | GEO

Chip-seq data of mouse Spermatogonia cells of Tet1-ko and WT

Project description:We analysed 2 samples of mouse spermatogonia cells including 3-month-old WT and 3 month-old Tet1

2020-01-18 | GSE140999 | GEO

Affymetrix gene expression array (mouse 2.0) of sorted undifferentiated spermatogonia in inducible Miwi2 knock-out model

Project description:Undifferentiated spermatogonia were sorted from Miwi2Tom/FL; ERT-Cre/+ (Miwi2-iKO) and Miwi2Tom/+; ERT-Cre/+ (Miwi2-CTL) mice injected with tamoxifen two weeks after last tamoxifen injection to understand an impact of acute Miwi2 deletion on spermatogenic homeostasis.

2018-03-21 | E-MTAB-6612 | biostudies-arrayexpress

RNA-seq data of Spermatogonia cells of Tet1-ko and WT

Project description:RNA-seq data of Spermatogonia cells of Tet1-ko and WT

| PRJNA588041 | ENA

Quantification of transcriptome levels in mouse spermatogonia TET1 overexpression group and control cells by RNA-seq

Project description:Quantification of transcriptome levels in mouse spermatogonia TET1 overexpression group and control cells by RNA-seq

| PRJNA797370 | ENA

RNA sequencing of ID4-GFP Bright and ID4-GFP Dim spermatogonia from postnatal day 8 transgenic Id4-Gfp (LT11) mouse pups and ID4-GFP+ spermatogonia from ID4 overexpression mouse model and control

Project description:Mice that constitutively overexpress ID4 in germ cells have impaired spermatogenic lineage development. The transcriptome of ID4-GFP+ spermatogonia from testes of ID4 overexpression animals was compared to the ID4-GFP+ population from controls

2017-01-20 | GSE93866 | GEO

Chip-seq data of mouse Spermatogonia cells of Tet1-ko and WT

Project description:Chip-seq data of mouse Spermatogonia cells of Tet1-ko and WT

| PRJNA591633 | ENA

10x Genomics Drop-seq single-cell RNA-seq of isolated Adult human spermatogonia, spermatocytes, spermatids & steady-state spermatogenic cells

Project description:To reveal distinct transcriptomes associated with various spermatogenic cells, including spermatogonial stem cells and all of their subsequent progeny, single-cell transcriptomes from Adult human spermatogonia, StaPut-enriched spermatocytes and spermatids, or unselected steady-state spermatogenic cells were used for Drop-Seq analysis. We used the 10x Genomics Chromium (Drop-Seq) to perform single-cell RNA-seq

2018-11-06 | GSE109037 | GEO

Spermatogonia & Buslfan

Project description:Spermatogonia & Buslfan

| PRJNA691760 | ENA