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Single-cell transcriptomic analysis of blood T cells from healthy controls, MS patients and COVID-19 patients by 10X genomics

ABSTRACT: In order to better understand the functional properties of this type of cells under different circumstances, we integrated the single-cell RNA-seq data of peripheral blood CD8+ T cells from healthy subjects, MS patients and COVID-19 patients generated from the 10X Genomics platform using the Seurat package. KIR+CD8+ T cells from different conditions (healthy, MS, and COVID-19) formed a distinct cluster with high expression of effector genes (GZMB and PRF1) as well as KIR transcripts. These findings reveal the commonality of KIR+CD8+ T cells across physiological and diseased status as well as their uniqueness relative to other CD8+ T cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE193770 | GEO | 2022/03/08

REPOSITORIES: GEO

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Transcriptional profiling of human KIR+ and KIR- CD8 T cells by bulk RNA-seq

Project description:Previous reports show that Ly49+CD8+ T cells can suppress autoimmunity in mouse models of autoimmune diseases. Here we find a markedly increased frequency of CD8+ T cells expressing inhibitory Killer cell Immunoglobulin like Receptors (KIR), the human equivalents of the Ly49 family, in the blood and inflamed tissues of various human autoimmune diseases. Moreover, KIR+CD8+ T cells can efficiently eliminate pathogenic gliadin-specific CD4+ T cells from Celiac disease (CeD) patients’ leukocytes in vitro. Furthermore, we observe elevated levels of KIR+CD8+ T cells, but not CD4+ regulatory T cells, in COVID-19 and influenza-infected patients, and correlate with disease severity and vasculitis in COVID-19. Expanded KIR+CD8+ T cells from these different diseases display shared phenotypes and similar T cell receptor sequences. These results characterize a regulatory CD8+ T cell subset in humans, broadly active in both autoimmune and infectious diseases, which we hypothesize functions to control self-reactive or otherwise pathogenic T cells.

2022-03-08 | GSE168527 | GEO

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2020-08-21 | E-MTAB-9221 | biostudies-arrayexpress

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