Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Whole genome sequencing of WT, ccrZ deletion, and overexpressed ccrZ in Bacillus subtilis

ABSTRACT: We obtained whole genome sequencing data as a measure of chromosome replication to see if deletion or overexpression of ccrZ altered replication initiation

ORGANISM(S): Bacillus subtilis

PROVIDER: GSE193981 | GEO | 2022/05/02

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Whole genome sequencing of overexpressed ccrZ and overexpressed ccrZ-D166A in Bacillus subtilis.

Project description:We obtained whole genome sequencing data as a measure of chromosome replication to see the effects of a phosphotransferase dead ccrZ (amyE::Pxyl-ccrZ-D166A, ccrZ-)

2025-06-24 | GSE206936 | GEO

Whole genome sequencing of vehicle control and hydroxyurea treated Bacillus subtilis cells

Project description:We obtained whole genome sequencing data as a measure of chromosome replication to see if HU inhibits replication compared to vehicle control.

2021-03-29 | GSE169591 | GEO

Whole genome sequencing of ccrZ-, yabA::cat, and ccrZ- yabA::cat in Bacillus subtilis.

Project description:We obtained whole genome sequencing data as a measure of chromosome replication to see the effects of combining ccrZ deletion with yabA deletion, as well as the effect of making a phosphotransferase dead ccrZ

2025-06-24 | GSE206937 | GEO

Bacillus subtilis

Project description:Genome-wide binding of replication initiation proteins in Bacillus subtilis

| PRJNA130913 | ENA

Bacillus subtilis remains translationally active after CRISPRi-mediated replication initiation arrest

Project description:Initiation of bacterial DNA replication takes place at the origin of replication (oriC), a region characterized by the presence of multiple DnaA boxes that serve as the binding sites for the master initiator protein DnaA. The absence or failure of DNA replication can result in bacterial cell growth arrest or death. Here, we aimed to uncover the physiological and molecular consequences of stopping replication in the model bacterium Bacillus subtilis. For this purpose, DNA replication was blocked using a CRISPRi approach specifically targeting DnaA boxes 6 and 7, which are essential for replication initiation. We characterized the phenotype of these cells and analyzed the overall changes in the proteome using quantitative mass spectrometry. Cells with arrested replication were elongating and not dividing but showed no evidence of DNA damage response (DDR). Moreover, these cells did not cease translation over time. This study sets the ground for future research on non-replicating but translationally active B. subtilis, which might be valuable for biotechnological applications.

2024-03-26 | PXD036876 | Pride

Whole genome sequencing of WT, ccrZ deletion, and overexpressed ccrZ in Bacillus subtilis

Project description:Whole genome sequencing of WT, ccrZ deletion, and overexpressed ccrZ in Bacillus subtilis

| PRJNA798681 | ENA

Genome-wide binding of replication initiation proteins in Bacillus subtilis

Project description:Initiation of DNA replication requires binding of the initiator protein, DnaA, to specific binding sites in the chromosomal origin of replication, oriC. In low G+C Gram-positive bacteria, the primosomal proteins DnaD and DnaB, in conjunction with loader ATPase DnaI, load the replicative helicase at oriC, and this depends on DnaA. DnaD and DnaB are also required to load the replicative helicase outside of oriC during replication restart, in a DnaA-independent manner. DnaA also binds to many sites around the chromosome, outside of oriC, and acts as a transcription factor at several of these. Using chromatin immunoprecipitation, we found that DnaD and DnaB, but not the replicative helicase, are associated with many of the chromosomal regions bound by DnaA in vivo in Bacillus subtilis. This association was dependent on DnaA and the order of recruitment was the same as that at oriC, but was independent of a functional oriC. The presence of DnaD and DnaB at the secondary (non-oriC) targets of DnaA in the absence of helicase loading indicates a possible role for DnaD and DnaB in modulating the activity of DnaA.

2010-12-07 | GSE23686 | GEO

Genome-wide binding of replication initiation proteins in Bacillus subtilis

2010-12-07 | E-GEOD-23686 | biostudies-arrayexpress

Bacillus subtilis

Project description:DnaN causes overinitiation of DNA replication

| PRJNA123527 | ENA

Whole-genome sequences of Bacillus subtilis and close relatives.

Project description:We sequenced four strains of Bacillus subtilis and the type strains for two closely related species, Bacillus vallismortis and Bacillus mojavensis. We report the high-quality Sanger genome sequences of B. subtilis subspecies subtilis RO-NN-1 and AUSI98, B. subtilis subspecies spizizenii TU-B-10(T) and DV1-B-1, Bacillus mojavensis RO-H-1(T), and Bacillus vallismortis DV1-F-3(T).

| S-EPMC3347079 | biostudies-literature

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data